Prof. I.S. Bright Singh, FNAAS

Founder and Emeritus Professor

Prof. I.S. Bright Singh, Founder, National Centre for Aquatic Animal Health, Cochin University of Science Technology specializes in Preventive Health Care in Aquaculture. He has served as Director, School of Environmental Studies, Dean Faculty of Environmental Studies, and UGC – BSR Faculty at National Centre for Aquatic Animal Health. Right now he is KSCSTE Emeritus Scientist. He conceptualized M.Tech. Marine Biotechnology programme sponsored by the Department of Biotechnology, Government of India offered by Cochin University of Science and Technology and served as the Course Coordinator from 2008 to 2017. He was elected as Fellow to National Academy of Agricultural Sciences (ICAR) on 2014 and awarded Dr. S. Jones Memorial Prize for outstanding Marine Biologist of India, Instituted by Marine Biological Association of India on 2014.

Graduation from Kerala University, Kerala (1977); Post graduation from CAS in Marine Biology, Annamalai University, Tamil Nadu (1977-1979); Ph.D. from School of Marine Sciences, Cochin University of Science and Technology (1988) for the thesis entitled ‘Studies on the bacteria associated with Penaeus indicus in a culture system’.

Participation in various Expert Committees

(constituted by Govt. of India, Govt. of Kerala/ University)

  1. QRT Member to ICAR – Central Institute of Fisheries Education, Mumbai.
  2. DBT nominee – Institute Biosafety Committee (IBSC), Indian Institute of Spices Research, Indian Council of Agricultural Research, Govt. of India.
  3. Member – Task Force in Aquaculture and Marine Biotechnology, Department of Biotechnology, Government of India.
  4. Member – Task Force for Fish and Shellfish Health Management constituted by Indian Council of Agricultural Research, Government of India.
  5. Aquaculture Expert Committee constituted to suggest creative recommendations for the scientific improvement of Poyya Prawn Farm and Training Center, Thrissur, Kerala. Status-Member.
  6. Governing body of State Fisheries Resource Management Society, Government of Kerala. Status-Member.
  7. High Power Committee constituted by Government of Kerala, to strengthen disease management and preventive measures to protect shrimp culture from viral diseases. Status-Member.
  8. High level committee on Aquaculture Drug Regulations constituted by CFDDM, CUSAT and Aquaculture Authority, Government of India. Status-Convener.
  9. Member expert group to suggest both short-term and long-term measures for creating an appropriate and effective legal and institutional framework for management and control of aquatic animal diseases- Govt. of India , Ministry of Agriculture.
  10. Member, Research advisory committee of Marine BioResource Centre (MBRC), Govt. of Gujarat.
  11. Member –Fisheries –Aquaculture Policy- Committee for framing draft Aquaculture policy of Government of Kerala.
  12. Member – Fish Health Management- Disease monitoring, Control and surveillance (MCS) Committee constituted by Department of Fisheries, Government of Kerala.

Awards/prizes/certificates

  • Life Time Achievement Award – for the contributions to Teaching, Research and Services to aquaculture Industry on the occasion of 80th Anniversary celebrations of School of Marine Sciences, CUSAT, MARICON,16-20, December, 2019.
  • Certificate of Excellence by Fisheries Station, Puduveypu, Kerala University of fisheries and Ocean Studies on 16th November, 2016 during the mangrove Festival, 2016.
  • UGC – BSR Faculty Fellowship (2015-2018)
  • Dr. S. Jones Memorial Prize – 2014 for outstanding contributions in Marine Biology and Aquatic Animal health, instituted by Marine Biological Association of India.
  • Fellow, National Academy of Agricultural Sciences, ICAR, New Delhi.
  • UGC – BSR One time grant of Rs. 7,00,000.0(Mid term career Award).
  • Regional Expert of NACA in Shrimp Diseases.
  • Visiting Professor in Microbiology, University of Northumbria, New Castle upon Tyne, United Kingdom (2001 to 2008).
  • UGC Visiting Associateship for the year 1993 to 1995 to work on the development of new fish cell lines using the expertise available with the Department of Microbiology, Sree Chithra Thirunal Institute of Medical Science and Technology, Trivandrum.
  • Young Scientist Award in Fisheries (1989) by State Committee on Science, Technology and Environment for the paper entitled “Bacteriology of the eggs and larvae of Penaeus indicus in hatchery” presented at the first Kerala Science Congress. The award comprised a citation, a cash award of Rs.5,000 and a research grant of Rs.20,000/- for a period of two years to continue the study in the same line.
  • Good Service Entry by Kerala Agricultural University for successfully conducting a training programme in the Hygienic Handling of Prawn/fishes for peeling shed workers.

Teaching & Academic Activities

Junior Assistant Professor, Regional Rice Research Station, Kerala Agricultural University, Vytilla, Cochin (1983-89)

Courses Offered: Fishery Microbiology and Fish Pathology at B.Fsc. and M. Fsc. Level at College of Fisheries, Kerala Agricultural University, Panangad. 

Lecturer in Environmental Microbiology, School of Environmental Studies, CUSAT

Courses Offered: Environmental Microbiology, Applied Environmental Microbiology, Environmental Biotechnology and Bioremediation at M.Sc. level.

Course Coordinator, M.Tech Marine Biotechnology Programme, National Centre for Aquatic Animal Health, CUSAT

Courses Offered: High Health Broodstock Development,Marine Microbiology and Biotechnological Interventions in Aquatic Animal Health Management at  M.Tech. Level

DBT Skill Vigyan Programme under State Partnership in Life Science and Biotechnology.

Conceptualized and proposal submitted to DBT for

  1. Technician training -Aquatic Animal Health Manager/Entrepreneur
  2. Entrepreneurship Training in Bioprocess Engineering
  3.  Finishing School in Biotechnology (Marine) with focus on Aquaculture Medicine and Aquatic Animal Health Management

Extension Activities – Current

The activities are being carried out through the extension cell of NCAAH which is supported by two Project Scientists, one Aquatic Animal Health Assistant, and two junior aquatic Animal Health Assistants under my Coordinatorship. The programme is financially supported with revenue generated. The major areas of Endeavour are:

  1. Disease Diagnostic services
  2. Preventive health care in aquaculture – Regulation of water and sediment quality, gut probiotic and immunomodulators.
  3. Zero water exchange aquaculture system – in earthen ponds managed through bioremediators
  4. Recirculating aquaculture systems – as Backyard RAS at National level. Eighty five such units have been already constructed
  5. Integrated multi-trophic aquaculture systems – Initial Stage of development

Major Extension Activity

Establishment of Land based Backyard RAS for fish culture

Innovative project on High Density Fish Culture under RAS Mode Integrated with Vegetable Cultivation Designated as “Backyard RAS”

Ongoing Doctoral Programmes

  1. Polyhydroxybutyrate from marine bacteria and their application in human and veterinary medicine – Linu Balan.
  2. Development and production of plant derived saponins as effective prophylactic agents of white spot syndrome virus in shrimp culture -Ahna Ameer
  3. Metabolic engineering in Penaeus monodon to accomplish maturation and spawning under captive breeding by way of RNA mediated silencing of CMG family hormone genes – Gopika Venu
  4. Development of cell culture systems from marine molluscs – Sowmya Balakrishnan
  5. Pyocyanin as drug of choice in aquaculture: Synthetic biology based production, downstream process, product design and application – Jini Jimmy
  6. Preventive health care and growth enhancement in Macrobrachium rosenbergii from hatchery to grow out by way of gut microbiome manipulation – Vinusree K.P.

Professional Experience

  • KSCSTE Emeritus Scientist (2018-21)
  • UGC, BSR Faculty (2015-18 )
  • Professor, School of Environmental Studies, CUSAT (2004-15)
  • Coordinator, National Centre for Aquatic Animal Health (2000-15)
  • Dean, Faculty of Environmental Studies (2007-10)
  • Director, School of Environmental Studies, CUSAT (2006-09)
  • Reader, School of Environmental Studies CUSAT (1996-04)
  • Senior Lecturer, School of Environmental Studies, CUSAT (1991-96)
  • Asst.Professor, College of Fisheries Kerala Agricultural University (1989-91)
  • Jr. Asst. Professor, College of Fisheries, Kerala Agricultural University (1983-89)

Contributions

  1. Packed Bed Nitrifying Bioreactor
  2. Stringed bed suspended nitrifying bioreactor
  3. Trickling Nitrifying bioreactor
  4. Nitrifying bacterial consortia
  5. Detritus bioaugmentor (Detrodigest)
  6. Gut probiotics
    1. PS -1: Pseudomonas aeruginosa MCCB 102
    2. Enterotrophotic – a blend of Bacillus cereus MCCB101 ( Genbank No. EF062509), Arthrobactor nicotianae MCCB 104 (Genbank No. EU402968)
  7. Zero water exchange shrimp culture technology- This technology is principally based on bioremediation of detritus, ammonia and hydrogen supplied by way of both bio-stimulation and bio-augmentation. In this methods water is not exchanged instead the loss due to evaporation and seepage is compensated by letting the water in.
  8. Nitrifying bacterial consortia immobilized in wood power
    This technology provides avenue for establishment of nitrification instantaneously in finfish and shellfish culture systems.
  9. Bioremediation of hydrogen sulphide
    The three selected bacterial strains can be used as bio-augmenters in aquaculture wherever there is hydrogen sulphide toxicity and extremely low Eh.
  10. Bioprocess Technology for the Production of Antagonistic Probiotic Pseudomonas MCCB 102 Using Response Surface Methodology
  11.  Bioprocess Technology for the Production of Antagonistic Probiotic Pseudomonas MCCB 103 Using Response Surface Methodology
  12. Bioprocess Technology for the Production of Antagonistic Probiotic Arthrobacter nicotiana MCCB 104 Using Response Surface Methodology
  13.  Immunostimulant –Acremonium dyosporii – as a source material for ß– 1,3 glucan
  14. Inactivated Virus Preparation (IVP) against WSSV in shrimp – This is WSSV vaccine ready for integration to zero water exchange shrimp culture.
  15. Vibrio harveyi phages in shrimp hatcheries as therapeutics – ready for application in shrimp hatcheries
  16. Chitosan based delivery system for aquaculture drugs to the cultured species.
  17. dsRNA based silencing of CMG family hormone genes in shrimp for maturation – They are at the level of standardization under laboratory conditions.
  18. Backyard RAS.
  19. World’s first shrimp – insect hybrid cell line PmLyO – Sf.
  20. Host specific (crustacean) baculovirus expression system.

Projects Undertaken

Cell and developmental biology of Marine organisms

Funding Agency: Department of Biotechnology, Govt of India

Project Type: Multi Institutional

Period of Study: 02/09/21 to 01/09/24

Project Coordinator: Prof.I. S. Bright Singh

PI: Prof. Valsamma Joseph and Dr. Jayesh Puthumana

Project Cost: Rs. 801.056 lakhs

Collaborating Institutions: 1. C Abdul Hakeem College, Vellore
                                                              2. Bharathidasan University, Thiruchirapalli

Development of an appropriate delivery system for dsRNA of CMG family hormone genes to effect maturation and spawning of Penaeus monodon under captivity

Funding Agency: KSCSTE Emeritus Scientist Scheme
Project Cost: Rs. 20, 82,000.0.

M1. Evaluating costs and benefits of prophylactic health products and novel alternatives on small holder aquaculture farmers in Asia and Africa (IMAQualate)

Funding Agency: Department of Biotechnology, Government of India,
Project Cost: Rs. 55.232.

Indo -US initiatives on innovative reforms in marine biotechnology education and research, and development of sustainable aquaculture production system for inclusive economic growth and sustainable development – under Indo – US 21st Century knowledge initiative

Funding Agency: University Grants Commission, New Delhi
Period of Study: 1st August 2015 to 31st July, 2018
Project Cost: Rs. 125.29.

Metabolite engineering in Penaeus monodon to accomplish maturation and spawning under captivity by way of RNA mediated silencing of CMG family hormones genes

Funding Agency:  UGC – BSR faculty Fellowship, University Grants Commission, New Delhi;
Period of Study: 2nd May 2015 to 1st May 2018
Project Cost: Rs. 25.74.

Selection and identification of immunocompetent early life stages of the giant fresh water prawn Macrobrachium rosenbergii based on differential mRNA expression.

Period of Study: 19-6-2013 to 18-6-2016
Funding Agency:Department of Science and Technology, Government of India
Project Cost:Rs. 43.67 lakhs

Detailed performance evaluation and accelerated commercialization of the nitrifying bioreactor technology in Indian market – Phase II.

Funding Agency: Department of Biotechnology, Government of India, Under SBIRI Scheme
Period of Study: 7-11-2013 to 7-11- 2016
Project Cost: Rs. 15.0 lakhs

Polyhydroxy butyrate from marine microorganisms for biomedical and aquaculture applications.

Funding Agency: Centre for Marine Living Resources and Ecology, Ministry of Earth Science, Government of India
Period of Study: 1-4-2012 to 31-3-2017
Project Cost: Rs.87.0 Lakhs.

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Immortalization of lymphoid cell culture from Penaeus monodon by transduction with oncogenes, ectopic expression of telomerase reverse transcriptase using shrimp specific expression vectors and hybridization with established cell lines.

Funding Agency: Department of Biotechnology, Government of India,
Period of Study:14-9-2012 to 13-9- 2015
Project Cost: Rs. 46. 07 Lakhs.

Development and optimization of vaccine to protect shrimp from white spot syndrome virus

Funding Agency: Department of Biotechnology, Government of India,
Period of Study: 24 -8-2009 to 31-3-2014
Project Cost: Rs. 57.03 Lakhs

Metagenomic approach to cloning and expression of endo and exo glucanase and β- 1,4 glucosidase from soil water system

Funding Agency: Department of Biotechnology, Government of India,
Period of Study: 28-9-2010 to 27-9-2014
Project Cost: Rs. 40.02 lakhs.

Optimization of the bioremediation technology developed for shrimp culture under closed system mode

Funding Agency: Ministry of Earth Science, Government of India,
Period of Study: October 2008 to October2011
Project Cost: Rs. 23.832 lakhs 

Development of anti-WSSV drugs from mangrove plants endemic to Indian coastal zone

Funding Agency: Department of Biotechnology, Government of India,
Period of Study: October 2006 –September 2009
Project Cost: Rs. 37.45 Lakhs.

Expression of virulence of Vibrio harveyi in Organic Recirculation Prawn Seed Production System (ORPSPS) and its management

Funding Agency: UGC,
Period of Study: July 2007-June 2010
Project Cost: Rs. 9.276 Lakhs.

Design modification and commercialization of nitrifying bioreactor technology for the establishment of organic recirculation prawn seed production system (Phase- I)

Funding Agency: Department of Biotechnology, Government of India,
Period of Study: September 2007-August 2010
Project Cost: Rs. 10.37 lakhs. 

Identification and removal of blocks on in vitro transformation and establishment of cell lines from Penaeus monodon

Funding Agency: Department of Biotechnology, Government of India,
Period of Study: October 2007- September 2010
Project Cost: Rs. 31.76 lakhs. 

Development and amplification of CMG family recombinant hormones, their antagonists and RNAi technique for induced maturation and spawning of Penaeus monodon

Funding Agency: Department of Biotechnology, Government of India,
Period of Study: 28-3-2005 to 31-12-2008
Project Cost: Rs. 38.69 lakhs. 

Programme support in marine biotechnology.

Funding Agency: Department of Biotechnology, Government of India,
Period of Study: April 2005- September 2008
Project Cost: Rs.139 lakhs.

Nitrifying bioreactors integrated into prawn hatchery systems: Biofilm structure, population dynamics, and kinetic modeling.

Funding Agency: Department of Science and Technology (DST), Govt. of India.
Period of Study: August 2006- March 2009
Project Cost: Rs. 13.9 lakhs.

Diseases of ornamental fishes: aetiology, disease management and development of biosecurity packages for exotic species.

Funding Agency: The Marine Products Export Development Authority (MPEDA), Ministry of Commerce, Government of India.
Period of Study: May 2007
Project Cost: Rs 8.59625 lakhs.

Development of an appropriate technology for bioremediation in shrimp grow out systems for disease management, productivity and aquaculture environment protection

Funding Agency: Indian Council of Agricultural Research (ICAR), New Delhi
Period of Study: 2003 October – 2006 October
Project Cost: Rs. 15,87,616.0.

Development of a comprehensive microbial technology for the protection of the larvae of Macrobrachium rosenbergii from vibriosis in hatchery.

Funding Agency: Department of Science and Technology (DST), Govt. of India
Period of Study: November 2001 to November 2004
Project Cost: Rs. 15, 06, 651.00.

Development of Microbial Technology for exclusion of vibrios from prawn larval rearing systems.
Development of cell culture systems from penaeid prawns for the isolation of White Spot Virus.

Funding Agency: Department of Biotechnology, Govt. of India.
Period of Study: November 1999 to March 2003
Project Cost: Rs. 19, 94, 000.00.

Demonstration and validation of the nitrifying bioreactors developed for penaeid and non penaeid closed hatchery systems.

Funding Agency: Department of Biotechnology, Govt. of India
Period of Study: July 2001 to March 2003
Project Cost: Rs. 3,93,400.00

Systemic Ectodermal Mesodermal Baculovirus Disease in Penaeids – Epizootiology, Immunodiagnostics and vaccine.

Funding Agency: Indian Council of Agricultural Research
Period of Study: April 1998 to April 2002
Project Cost: Rs. 11, 75,576.00.

Screening of commercially available aquaculture medicine for the management of luminescent vibrios in penaeid hatchery systems

Funding Agency: Marine Products Export Development Authority of India
Period of Study: June 2001 to Nov 2001
Project Cost: Rs. 2, 21, 951.0

Development of Disease Diagnosis and Management Strategies in Finfish and Shellfish Culture Systems.

Funding Agency: Department of Fisheries, Govt. of Kerala
Period of Study: August 1998 to July 2001
Project Cost: Rs.10,00,000.00.

Development of primary cell cultures and cell lines from fresh water fishes to isolate viruses associated with EUS.

Funding Agency: State Committee on Science, Technology & Environment, Govt. of Kerala
Period of Study: January 1993 to January 1998
Project Cost: Rs. 2,17,150.00.

Development of bioreactors for nitrifying water in closed system prawn hatcheries.

Funding Agency: Department of Biotechnology, Govt. of India.
Period of Study: April 1995 to April 1998
Project Cost: Rs. 19,31,944.00

Coir Retting – Process upgradation and pollution abatement through Environmental Biotechnology.

Funding Agency: Coir Board
Period of Study: August 1993 to April 1998
Project Cost: Rs.5,99,200.0

Vibrionaceae associated with the larvae of Macrobrachium rosenbergii in hatchery – Systematics and pathogenicity.

Funding Agency: International Foundation for Science, Sweden
Period of Study: February 1990 to February 1995
Project Cost: US $ 9,600.00.

Vibrionaceae associated with the larvae of Penaeus indicus: Systematics and Pathogenicity.

Funding Agency: State Committee on Science, Technology & Environment. Govt. of Kerala. (Part of the Young Scientist award)
Period of Study: August 1990 to July 1992
Project Cost: Rs. 20,000.00.

Microorganisms associated with the eggs and larvae of Macrobrachium rosenbergii.

Funding Agency: Department of Science and Technology, Govt. of India, under the Young Scientist programme
Period of Study: January 1987 to July 1989
Project Cost: Rs. 98,000.0

Molecular screening, cell culture based isolation and characterization of finfish and shellfish viruses and establishment of National Repository

Funding Agency: Department of Biotechnology, Government of India,
Project Type: Multi-institutional research project,

Period of Study: 11-07-2017 to 10-07-2020

Project Cost: Rs. 118. 663 lakhs.

Marine synthetic biology: building national capacity and human resource.

Funding Agency: Department of Biotechnology, Government of India,
Project type: Multi-Institutional
Period of Study:28-2-2017 to 27-2-2020
Project Cost: Rs. 51.49884.

Development and application of CMG family recombinant hormones, their antagonists and RNAi technique for induced maturation and spawning of Penaeus monodon.

Collaborators:  Central Institute of Brackishwater Aquaculture, Chennai
                                    Department of Zoology, Calicut University, Calicut.

Funding Agency: Department of Biotechnology, Govt. of India.

Period of Study: October 2005- September 2008,

Project Cost: Rs. 66.03 lakhs.

Development of appropriate bioremediation technology for zero-exchange creek-fed shrimp culture system.

Collaborator: M/s Pancham Aquaculture Limited, Mumbai.
Funding Agency: Department of Biotechnology, Govt. of India under the Institute-Industry Collaboration Programme.
Period of Study: September 2004 – September 2007
Project Cost: Rs. 19,38,000.00.

Detection, isolation and identification of anti-white spot syndrome virus molecules from selected species of marine biota and development of an anti-WSSV formulation.

Collaborator: National Centre for Cell Sciences, Pune.

Funding Agency: Department of Biotechnology, New Delhi.

Period of Study: 2003 September – 2005 September

Project Cost: Rs. 11, 65, 000.00.

Development and evaluation of small-scale solar treatment systems for contaminated drinking water.

Collaborator: University of Northumbria at Newcastle, U.K.
Funding Agency: The Wellcome Trust, UK.
Period of Study: November 2001 to June 2005,
Project Cost: UK Pounds 53,380.00.

Digitized inventory of marine bioresources with special reference to bacteria associated with marine plants and animals.

Collaborators: Multi-Institutional Consortium consisting of National Institute of Oceanography, Goa, Andhra University, Visakhapatnam, Annamalai University, Porto Novo and Cochin University of Science and Technology, Kochi.
Funding Agency: Department of Biotechnology and Department of Ocean Development, Govt. of India.
Period of Study: November 2001 to March 2003
Project Cost: Rs. 10,87,000.00.

Publications

  1. Jayesh P and I.S Bright Singh (2014) Advances in the Development of Shrimp Cell Lines ISBN 978-93-80095-54-7. Directorate of Public Relations and Publications, CUSAT, India.
  2. Singh, I.S.B., Y. S. Yadava (Editors), (2016). Aquaculture Medicine and Aquatic Animal Health Management (ISBN 81-901701-0-4). Aquaculture Authority, Government of India, Chennai.
  3. Singh, I.S.B., Joseph V, Phillip R, and Mohandas A (Editors).2007. Aquaculture and Marine Biotechnology.(ISBN.81-900724-2-0).
  4. Editorial board member, 2007. Fisheries and Aquaculture: Strategic outlook for Asia. Book of Abstracts, 8th Asian Fisheries Forum. (ISBN: 81-85340-21-8).Asian Fisheries Society, India.
  5. Singh, I.S.B., S. Somnath Pai, R. Phillip, A. Mohandas (Editors), 2003. Aquaculture Medicine (ISBN 81-900724-1-2). Centre for Fish Disease Diagnosis and Management, Cochin University of Science and Technology, Cochin.
  6. Mohandas A., I.S. Bright Singh (Editors), 1998. Frontiers in Applied Environmental Microbiology (ISBN 81-900724-0-4). School of Environmental Studies, Cochin University of Science and Technology, Cochin.
  1. Singh ISB, Kumar VJR, Achuthan C, Manju NJ, Philip R (2007) Recirculation zero water exchange system for hatchery rearing of economically important marine organisms. In: Vijayan KK, Jayasankar P, Vijayagopal P (Eds) Indian Fisheries- A Progressive Outlook. Central Marine Fisheries Institute, Kochi, India. p139-155.
  2. Singh ISB, Yadava YS, Pai SS (2003) Aquaculture drug regulations in the Indian context. In: Singh ISB, Pai SS, Philip R, Mohandas A (eds) Aquaculture Medicine (ISBN 81-900724-1-2), Centre for Fish Disease Diagnosis and Management, CUSAT, Kochi, India. p323–332.
  3. Anas A, Singh ISB, Jayaprakash NS (2003) Microencapsulation as a technique for drug targeting. In: Singh ISB, Pai SS, Philip R, Mohandas A (eds) Aquaculture Medicine (ISBN 81-900724-1-2), Centre for Fish Disease Diagnosis and Management, CUSAT, Kochi, India. p293-298.
  4. Philip R, Sajeevan TP, Singh ISB (2003) Crustacean defense mechanisms and the concept of vaccinating shrimps. In: Singh ISB, Pai SS, Philip R, Mohandas A (eds) Aquaculture Medicine (ISBN 81-900724-1-2), Centre for Fish Disease Diagnosis and Management, CUSAT, Kochi, India. p 203 – 210.
  5. Singh ISB, Anas A (2003) Preparation of bacterins for use as prophylactic tools for protecting shellfishes from bacteraemia. In: Singh ISB, Pai SS, Philip R, Mohandas A (eds) Aquaculture Medicine (ISBN 81-900724-1-2), Centre for Fish Disease Diagnosis and Management, CUSAT, Kochi, India. p198–202.
  6. Singh ISB, Achuthan C, Kumar VJR, Manju NJ, Philip R (2003) Bioreactor technology for rapid removal of ammonia and nitrite from prawn larval rearing systems. In: Singh ISB, Pai SS, Philip R, Mohandas A (eds) Aquaculture Medicine (ISBN 81-900724-1-2), Centre for Fish Disease Diagnosis and Management, CUSAT, Kochi, India. p133–134.
  7. Ranjit S, Singh ISB (2003). Antialgal compounds – their significance and application in shrimp aquaculture. In: Singh ISB, Pai SS, Philip R, Mohandas A (eds) Aquaculture Medicine (ISBN 81-900724-1-2), Centre for Fish Disease Diagnosis and Management, CUSAT, Kochi, India. p56–63.
  8. Preetha R, Singh ISB, Philip R (2003) Natural antiviral agents for aquaculture application. In: Singh ISB, Pai SS, Philip R, Mohandas A (eds) Aquaculture Medicine (ISBN 81-900724-1-2), Centre for Fish Disease Diagnosis and Management, CUSAT, Kochi, India. p49–55.
  9. Singh ISB, Kumar GS, Philip R (2003) Cell line as tolls for detection, isolation, and study of viruses rickettsias and other obligate pathogenic microbes of fish and prawns. In: Singh ISB, Pai SS, Philip R, Mohandas A (eds) Aquaculture Medicine (ISBN 81-900724-1-2), Centre for Fish Disease Diagnosis and Management, CUSAT, Kochi, India. p24–26.
  10. Pai SS, Singh ISB (2003) Nucleic acid based diagnostics for shrimp diseases. In: Singh ISB, Pai SS, Philip R, Mohandas A (eds) Aquaculture Medicine (ISBN 81-900724-1-2), Centre for Fish Disease Diagnosis and Management, CUSAT, Kochi, India. p13–23.
  11. Kumar GS, Singh ISB, Philip R (2001) A cell culture system from the eyestalk of Penaeus indicus. In: Animal Cell Technology: From Target to Market, E. Lindner-Olsson et al. (eds), p261-265.
  12. Bhat SG, Singh ISB (1998) Vibrionaceae associated with larval rearing system of Macrobrachium rosenbergii, systematics and pathogenicity. In: Flegel TW (ed) Advances in Shrimp Biotechnology: National Centre for Genetic Engineering and Biotechnology, Bangkok, p279

  1. Anju, M. V., Archana, K., Anooja, V. V., Athira, P.P., Neelima, S., Singh, I.S.B., Philip, R., 2023. A novel anti-lipopolysaccharide factor from blue swimmer crab Portunus pelagicus and its cytotoxic effect on the prokaryotic expression host, E. coli on heterologous expression. J. Genet. Eng. Biotechnol. 21, 22. https://doi.org/10.1186/s43141-023-00478-w view

  2. Sathyan, N., Muhammed Musthafa, S., Anju, M.V., Archana, K., Athira, P.P., Namitha, P., Chaithanya, E.R., Priyaja, P., Bright Singh, I.S., Rosamma, P., 2022. Functional characterization of a histone H2A derived antimicrobial peptide HARRIOTTIN-1 from sicklefin chimaera, Neoharriotta pinnata. Dev. Comp. Immunol. 104554. https://doi.org/10.1016/j.dci.2022.104554 view

  3. Athira, P.P., Anooja, V.V., Anju, M.V., Neelima, S., Archana, K., Muhammed Musthafa, S., Antony, S.P., Singh, I.S.B., Philip, R., 2022. A β-defensin isoform from the Flathead grey mullet, Mugil cephalus (Linnaeus 1758): Structural and functional characterisation in silico. Anim. Gene 25, 200128. https://doi.org/10.1016/j.angen.2022.200128 view

  4. Balakrishnan, S., Ameer, A., Pazhur Mohandas, S., Maliyekkal Sajeevan, A.K., Bhaskaran Sathyabhama, A., Singh, B., 2022. Pyocyanin as a safe aquaculture drug for the control of vibriosis in shrimp recirculating aquaculture system (RAS). Aquac. Int. 30, 2129–2144. https://doi.org/10.1007/s10499-022-00890-y view
  5. Balakrishnan, S., Singh, I.S.B., Puthumana, J., 2022. Status in molluscan cell line development in last one decade (2010–2020): impediments and way forward. Cytotechnology. https://doi.org/10.1007/s10616-022-00539-x [in press] view
  6. Ajitha, V., Sarasan, M., Parvathi Sreevidya, C., Aswathy, C., Kachiprath, B., Mohandas, A., Singh, I.S.B., Hameed, A.S.S., Schlenk, D., Magnuson, J.T., Puthumana, J., 2021a. Cytotoxic impacts of treated electroplating industrial effluent and the comparative effect of their metal components (Zn, Hg, and Zn+Hg) on Danio rerio gill (DrG) cell line. Sci. Total Environ. 793, 148533. https://doi.org/10.1016/j.scitotenv.2021.148533 view
  7. Ajitha, V., Sreevidya, C.P., Sarasan, M., Park, J.C., Mohandas, A., Singh, I.S.B., Puthumana, J., Lee, J.-S., 2021b. Effects of zinc and mercury on ROS-mediated oxidative stress-induced physiological impairments and antioxidant responses in the microalga Chlorella vulgaris. Environ. Sci. Pollut. Res. 28, 32475–32492. https://doi.org/10.1007/s11356-021-12950-6 view
  8. Anoop, B.S., Puthumana, J., Vazhappilly, C.G., Kombiyil, S., Philip, R., Abdulaziz, A., Bright Singh, I.S., 2021. Immortalization of shrimp lymphoid cells by hybridizing with the continuous cell line Sf9 leading to the development of ‘PmLyO-Sf9 .’ Fish Shellfish Immunol. 113, 196–207. https://doi.org/10.1016/j.fsi.2021.03.023 view
  9. Archana, K., Sebastian, S.R., Sruthy, K.S., Nair, A., Anju, M.V., Bright Singh, I.S., Philip, R., 2021. Molecular and Functional Characterization of an Anti-lipopolysaccharide Factor Mm-ALF from Speckled Shrimp Metapenaeus monoceros. Probiotics Antimicrob. Proteins 13, 1183–1194. https://doi.org/10.1007/s12602-021-09741-3 view
  10. B.S., A., Puthumana, J., Sukumaran, V., Vazhappilly, C.G., Kombiyil, S., Philip, R., Singh, I.S.B., 2021. A Novel Approach of Transducing Recombinant Baculovirus into Primary Lymphoid Cells of Penaeus monodon for Developing Continuous Cell Line. Mar. Biotechnol. 23, 517–528. https://doi.org/10.1007/s10126-021-10043-6 view
  11. Bhaskaran Sathyabhama, A., Puthumana, J., Kombiyil, S., Philip, R., Bright Singh, I.S., 2021. ‘PmLyO-Sf9 – WSSV complex’ could be a platform for elucidating the mechanism of viral entry, cellular apoptosis and replication impediments. Virology 553, 102–110. https://doi.org/10.1016/j.virol.2020.10.014 view
  12. Joseph, V., Chellappan, G., Aparajitha, S., Ramya, R.N., Vrinda, S., Rejish Kumar, V.J., Bright Singh, I.S., 2021. Molecular characterization of bacteria and archaea in a bioaugmented zero-water exchange shrimp pond. SN Appl. Sci. 3, 458. https://doi.org/10.1007/s42452-021-04392-z view
  13. Kumaran, J., Poulose, S., Joseph, V., Bright Singh, I.S., 2021a. Enhanced biomass production and proximate composition of marine microalga Nannochloropsis oceanica by optimization of medium composition and culture conditions using response surface methodology. Anim. Feed Sci. Technol. 271, 114761. https://doi.org/10.1016/j.anifeedsci.2020.114761 view
  14. Kumaran, J., Singh, I.S.B., Joseph, V., 2021b. Effective biomass harvesting of marine diatom Chaetoceros muelleri by chitosan-induced flocculation, preservation of biomass, and recycling of culture medium for aquaculture feed application. J. Appl. Phycol. 33, 1605–1619. https://doi.org/10.1007/s10811-021-02369-4 view
  15. Mohandas, S.P., Balan, L., Gopi, J., Anoop, B.S.S., Mohan P., S., Philip, R., Cubelio, S.S., Singh, I.S.B.S.B., Mohan P, S., Philip, R., Cubelio, S.S., Singh, I.S.B.S.B., 2021. Biocompatibility of polyhydroxybutyrate-co-hydroxyvalerate films generated from Bacillus cereus MCCB 281 for medical applications. Int. J. Biol. Macromol. 176, 244–252. https://doi.org/10.1016/j.ijbiomac.2021.02.006 view
  16. Neelima, S., Archana, K., Athira, P.P., Anju, M. V., Anooja, V. V., Bright Singh, I.S., Philip, R., 2021. Molecular characterization of a novel β-defensin isoform from the red-toothed trigger fish, Odonus niger (Ruppel, 1836). J. Genet. Eng. Biotechnol. 19, 71. https://doi.org/10.1186/s43141-021-00175-6 view
  17. Preena, P.G., Rejish Kumar, V.J., Singh, I.S.B., 2021. Nitrification and denitrification in recirculating aquaculture systems: the processes and players. Rev. Aquac. 13, 2053–2075. https://doi.org/10.1111/raq.12558 view
  18. Vijayan, A., Vattiringal Jayadradhan, R.K., Pillai, D., Prasannan Geetha, P., Joseph, V., Isaac Sarojini, B.S., 2021. Nitrospira as versatile nitrifiers: Taxonomy, ecophysiology, genome characteristics, growth, and metabolic diversity. J. Basic Microbiol. 61, 88–109. https://doi.org/10.1002/jobm.202000485 view
  19. Jose, D., Preena, P.G., Kumar, V.J.R., Philip, R., Singh, I.S.B., 2020. Metaproteomic insights into ammonia oxidising bacterial consortium developed for bioaugmenting nitrification in aquaculture systems. Biologia (Bratisl). 75, 1751–1757. https://doi.org/10.2478/s11756-020-00481-3 view
  20. Nair, R.R., Rangaswamy, B., Sarojini, B.S.I., Joseph, V., Boobal, R., Bright Singh, I.S., Joseph, V., 2020. Anaerobic ammonia-oxidizing bacteria in tropical bioaugmented zero water exchange aquaculture ponds. Environ. Sci. Pollut. Res. 27, 10541–10552. https://doi.org/10.1007/s11356-020-07663-1 view
  21. Preena, P.G., Swaminathan, T.R., Kumar, V.J.R., Singh, I.S.B., 2020. Antimicrobial resistance in aquaculture: a crisis for concern. Biologia (Bratisl). 75, 1497–1517. https://doi.org/10.2478/s11756-020-00456-4 view
  22. Preena, P.G., Swaminathan, T.R., Rejish Kumar, V.J., Bright Singh, I.S., 2020. Unravelling the menace: detection of antimicrobial resistance in aquaculture. Lett. Appl. Microbiol. 71, 26–38. https://doi.org/10.1111/lam.13292 view
  23. Sunish, K.S., Biji, M., Rosamma, P., Sudheer, N.S., Sreedharan, K., Mohandas, A., Singh, I.S.B., 2020. Marine actinomycetes Nocardiopsis alba MCCB 110 has immunomodulatory property in the tiger shrimp Penaeus monodon. Fish Shellfish Immunol. 102, 125–132. https://doi.org/10.1016/j.fsi.2020.03.069 view
  24. Ajitha, V., Sreevidya, C.P., Kim, J.H., Bright Singh, I.S., Mohandas, A., Lee, J.-S., Puthumana, J., 2019. Effect of metals of treated electroplating industrial effluents on antioxidant defense system in the microalga Chlorella vulgaris. Aquat. Toxicol. 217, 105317. https://doi.org/10.1016/j.aquatox.2019.105317 view
  25. Preena, P.G., Achuthan, C., Kumar, V.J.R., Boobal, R., Deepa, G.D., Puthumana, J., Poulose, S., Surekhamol, I.S., Singh, I.S.B., 2019. Community composition of marine and brackish water ammonia-oxidizing consortia developed for aquaculture application. Water Sci. Technol. 79, 1017–1028. https://doi.org/10.2166/wst.2019.029 view
  26. Sabu, S., Singh, I.S.B., Joseph, V., 2019. Improved lipid production in oleaginous brackish diatom Navicula phyllepta MACC8 using two-stage cultivation approach. 3 Biotech 9, 437. https://doi.org/10.1007/s13205-019-1968-1 view
  27. Sruthy, K.S., Nair, A., Antony, S.P., Puthumana, J., Singh, I.S.B., Philip, R., 2019a. A histone H2A derived antimicrobial peptide, Fi-Histin from the Indian White shrimp, Fenneropenaeus indicus: Molecular and functional characterization. Fish Shellfish Immunol. 92, 667–679. https://doi.org/10.1016/j.fsi.2019.06.044 view
  28. Sruthy, K.S., Nair, A., Antony, S.P., Puthumana, J., Singh, I.S.B., Philip, R., P, J., Singh, I.S.B., Philip, R., Puthumana, J., Singh, I.S.B., Philip, R., 2019b. A histone H2A derived antimicrobial peptide, Fi-Histin from the Indian White shrimp, Fenneropenaeus indicus: Molecular and functional characterization. Fish Shellfish Immunol. 92, 667–679. https://doi.org/10.1016/j.fsi.2019.06.044 view
  29. Vrinda, S., Abdulaziz, A., Abhilash, K.S., Jasmin, C., Kripa, V., Bright Singh, I.S., 2019. Neuroendocrine and immunotoxicity of polyaromatic hydrocarbon, chrysene in crustacean post larvae. Ecotoxicology. https://doi.org/10.1007/s10646-019-02094-2 view
  30. Babu, D.T., Archana, K., Kachiprath, B., Solomon, S., Jayanath, G., Singh, I.S.B., Philip, R., 2018. Marine actinomycetes as bioremediators in Penaeus monodon rearing system. Fish Shellfish Immunol. 75, 231–242. https://doi.org/10.1016/j.fsi.2018.01.037 view
  31. Jayanath, G., Mohandas, S.P., Kachiprath, B., Solomon, S., Sajeevan, T.P., Bright Singh, I.S., Philip, R., 2018. A novel solvent tolerant esterase of GDSGG motif subfamily from solar saltern through metagenomic approach: Recombinant expression and characterization. Int. J. Biol. Macromol. 119, 393–401. https://doi.org/10.1016/j.ijbiomac.2018.06.057 view
  32. Jose, D., Mohandas, A., Bright Singh, I.S., 2018a. Bioprocess technology for LasB protease (elastase) production from Pseudomonas aeruginosa MCCB 123. Indian J. Biotechnol. 17, 284–289 view
  33. Jose, D., Mohandas, A., Bright Singh, I.S., 2018b. A Non-Pathogenic Environmental Isolate of Pseudomonas aeruginosa MCCB 123 with Biotechnological Potential. Int. J. Curr. Microbiol. Appl. Sci. 7, 3060–3071. https://doi.org/10.20546/ijcmas.2018.701.363 view
  34. Kachiprath, B., Puthumana, J., Gopi, J., Solomon, S., Krishnan, K.P., Philip, R., 2018. Amplicon sequencing based profiling of bacterial diversity from Krossfjorden, Arctic. Data Br. https://doi.org/10.1016/j.dib.2018.11.101 view
  35. Mohan, A.S., Nusaira, P., Raju, R., Puthumana, J., Kachiprath, B., Solomon, S., Jayanath, G., Philip, R., 2018. Bacterial Communities from near Glacier and Transition Zones of Kongsfjorden, Arctic: A Metagenomic Study. Indian J. Sci. Res. 20, 56–62 view
  36. Nair, R.R., Boobal, R., Vrinda, S., Bright Singh, I.S., Valsamma, J., 2018. Ammonia-oxidizing bacterial and archaeal communities in tropical bioaugmented zero water exchange shrimp production systems. J. Soils Sediments. https://doi.org/10.1007/s11368-018-2185-y view
  37. Preena, P.G., Kumar, V.J.R., Achuthan, C., George, R., Boobal, R., Nair, R.R., Singh, I.S.B., 2018. Diversity of marine and brackish water nitrite-oxidizing consortia developed for activating nitrifying bioreactors in aquaculture. Int. J. Environ. Sci. Technol. 15, 2399–2410. https://doi.org/10.1007/s13762-017-1580-z view
  38. Vrinda, S., Jasmin, C., Philip, R., Bright Singh, I.S., 2018. Thioredoxin fused CHH1 protein as antigen for polyclonal antisera: Application to regulate glycemia in Penaeus monodon. Indian J. Exp. Biol. 56, 83–92. view
  39. Dhaneesha, M., Benjamin Naman, C., Krishnan, K.P., Sinha, R.K., Jayesh, P., Joseph, V., Bright Singh, I.S., Gerwick, W.H., Sajeevan, T.P., 2017. Streptomyces artemisiae MCCB 248 isolated from Arctic fjord sediments has unique PKS and NRPS biosynthetic genes and produces potential new anticancer natural products. 3 Biotech 7, 32. https://doi.org/10.1007/s13205-017-0610-3 view
  40. Jose, D., Jayesh, P., Gopinath, P., Mohandas, A., Bright Singh, I.S., 2017a. A rapid two step bacterial DNA extraction method using LasA protease of Pseudomonas aeruginosa MCCB 123. Indian J. Biotechnol. 16, 495–504. view
  41. Jose, D., Manjusha, K., Jose, S., Mohandas, A., Bright Singh, I.S., 2017b. Purification and characterization of highly active LasB protease from Pseudomonas aeruginosa MCCB 123. Indian J. Exp. Biol. 55, 303–310.view
  42. Kumaran, J., Jose, B., Joseph, V., Bright Singh, I.S., 2017. Optimization of growth requirements of marine diatom Chaetoceros muelleri using Response Surface Methodology. Aquac. Res. 48, 1513–1524. https://doi.org/10.1111/are.12987 view
  43. Preena, P.G., Manju, N.J., Deepesh, V., Thomas, A., Bright Singh, I.S., 2017. Genetic diversity of nitrate reducing bacteria in marine and brackish water nitrifying bacterial consortia generated for activating nitrifying bioreactors in recirculating aquaculture systems. Aquac. Res. 48, 5729–5740. https://doi.org/10.1111/are.13396 view
  44. Sabu, S., Bright Singh, I.S., Joseph, V., 2017a. Molecular Identification and Comparative Evaluation of Tropical Marine Microalgae for Biodiesel Production. Mar. Biotechnol. 19, 328–344. https://doi.org/10.1007/s10126-017-9754-8 view
  45. Sabu, S., Singh, I.S.B., Joseph, V., 2017b. Optimisation of critical medium components and culture conditions for enhanced biomass and lipid production in the oleaginous diatom Navicula phyllepta: a statistical approach. Environ. Sci. Pollut. Res. 24, 26763–26777. https://doi.org/10.1007/s11356-017-0274-x view
  46. Sukumaran, V., Jose, S., Chekidhenkuzhiyil, J., Lalithabai, M.D., Nair, S.B., Christo, D., Kizhakedath, V.K., Paran, B.C., Philip, R., Sarojini, B.S.I., 2017. ELISA detection of vitellogenin in the haemolymph of Penaeus monodon (Fabricius, 1798) (Caridea: Penaeidae): indirect indicator of female maturity. J. Crustac. Biol. 37, 473–480. https://doi.org/10.1093/jcbiol/rux040 view
  47. Vrinda, S., Jasmin, C., Sivakumar, K.C., Jose, S., Jose, B., Philip, R., Bright Singh, I.S., 2017. Regulating gonad inhibition and vitellogenin/vitellin induction in Penaeus monodon using mature GIH fusion protein and polyclonal antisera. Comp. Biochem. Physiol. Part A Mol. Integr. Physiol. 203, 167–178. https://doi.org/10.1016/j.cbpa.2016.09.007 view
  48. Afsal, V. V., Antony, S.P., Philip, R., Bright Singh, I.S., 2016. Molecular Characterization of a Newly Identified Subfamily Member of Penaeidin from two Penaeid Shrimps, Fenneropenaeus indicus and Metapenaeus monoceros. Probiotics Antimicrob. Proteins 8, 46–52. https://doi.org/10.1007/s12602-015-9203-9 view
  49. Arshad, E., Anas, A., Asok, A., Jasmin, C., Pai, S.S., Bright Singh, I.S., Mohandas, A., Biju, V., 2016. Fluorescence detection of the pathogenic bacteria Vibrio harveyi in solution and animal cells using semiconductor quantum dots. RSC Adv. 6, 15686–15693. https://doi.org/10.1039/C5RA24161H view
  50. Asha, P., Divya, J., Bright Singh, I.S., 2016. Purification and characterisation of processive-type endoglucanase and β-glucosidase from Aspergillus ochraceus MTCC 1810 through saccharification of delignified coir pith to glucose. Bioresour. Technol. 213, 245–248. https://doi.org/10.1016/j.biortech.2016.03.013 view
  51. Bright Singh, I.S., George, R., Jose, B., Sugunan, S., 2016. Preventive healthcare in Penaeus monodon under zero water exchange mode supported with bioaugmentors, probiotics and biological response modifiers. Fish Shellfish Immunol. 53, 61. https://doi.org/10.1016/j.fsi.2016.03.050 view
  52. Jayesh, P., Vrinda, S., Priyaja, P., Philip, R., Singh, I.S.B., 2016. Impaired telomerase activity hinders proliferation and in vitro transformation of Penaeus monodon lymphoid cells. Cytotechnology 68, 1301–1314. https://doi.org/10.1007/s10616-015-9890-9 view
  53. Priyaja, P., Jayesh, P., Haseeb, M., Jose, B., Philip, R., Singh, I.S.B., 2016a. Evaluation of pyocyanin toxicity in various life stages of Penaeus monodon and in nitrifying bacterial consortia for their safe application in recirculating aquaculture systems (RAS) to abrogate pathogenic vibrios. Aquac. Int. https://doi.org/10.1007/s10499-016-0075-0 view
  54. Priyaja, P., Jayesh, P., Philip, R., Bright Singh, I.S., 2016b. Pyocyanin induced in vitro oxidative damage and its toxicity level in human, fish and insect cell lines for its selective biological applications. Cytotechnology 68, 143–155. https://doi.org/10.1007/s10616-014-9765-5 view
  55. Puthumana, J., Philip, R., Bright Singh, I.S., 2016. Transgene expression in Penaeus monodon cells: evaluation of recombinant baculoviral vectors with shrimp specific hybrid promoters. Cytotechnology 68, 1147–1159. https://doi.org/10.1007/s10616-015-9872-y
  56. Sivakumar, K.C., Sajeevan, T.P., Bright Singh, I.S., 2016. Marine derived compounds as binders of the White spot syndrome virus VP28 envelope protein: In silico insights from molecular dynamics and binding free energy calculations. Comput. Biol. Chem. 64, 359–367. https://doi.org/10.1016/j.compbiolchem.2016.08.006 view
  57. Solomon, S., Kachiprath, B., Jayanath, G., Sajeevan, T.P., Bright Singh, I.S., Philip, R., 2016. High-quality metagenomic DNA from marine sediment samples for genomic studies through a preprocessing approach. 3 Biotech 6, 160. https://doi.org/10.1007/s13205-016-0482-y view
  58. Thomas, A., Sudheer, N.S., Kiron, V., Bright Singh, I.S., Narayanan, R.B., 2016. Expression profile of key immune-related genes in Penaeus monodon juveniles after oral administration of recombinant envelope protein VP28 of white spot syndrome virus. Microb. Pathog. 96, 72–79. https://doi.org/10.1016/j.micpath.2016.05.002 view
  59. Vrinda, S., Jasmin, C., Sivakumar, K.C., Jose, B., Philip, R., Bright Singh, I.S., 2016. Moult-inhibiting fusion protein augments while polyclonal antisera attenuate moult stages and duration in Penaeus monodon. Gen. Comp. Endocrinol. 233, 32–42. https://doi.org/10.1016/j.ygcen.2016.05.012 view
  60. Jayesh, Puthumana, Jose, S., Philip, R., Bright Singh, I.S., 2015. Cellular and molecular markers in monitoring the fate of lymphoid cell culture from Penaeus monodon Fabricius (1798). Fish Shellfish Immunol. 47, 893–901. https://doi.org/10.1016/j.fsi.2015.05.033 view
  61. Jayesh, P., Philip, R., Singh, I.S.B., 2015. Multifactorial interaction of growth factors on Penaeus monodon lymphoid cells and the impact of IGFs in DNA synthesis and metabolic activity in vitro. Cytotechnology 67, 559–571. https://doi.org/10.1007/s10616-014-9697-0 view
  62. Preetha, R., Vijayan, K.K., Jayaprakash, N.S., Alavandi, S. V., Santiago, T.C., Bright Singh, I.S., 2015. Optimization of Culture Conditions for Mass Production of the Probiotics Pseudomonas MCCB 102 and 103 Antagonistic to Pathogenic Vibrios in Aquaculture. Probiotics Antimicrob. Proteins 7, 137–145. https://doi.org/10.1007/s12602-015-9185-7 view
  63. Puthumana, J., Prabhakaran, P., Philip, R., Singh, I.S.B., 2015. Attempts on producing lymphoid cell line from Penaeus monodon by induction with SV40-T and 12S EIA oncogenes. Fish Shellfish Immunol. 47, 655–663. https://doi.org/10.1016/j.fsi.2015.08.010 view
  64. Sruthy, K.S., Chaithanya, E.R., Sathyan, N., Nair, A., Antony, S.P., Bright Singh, I.S., Philip, R., 2015a. Molecular Characterization and Phylogenetic Analysis of Novel Isoform of Anti-lipopolysaccharide Factor from the Mantis Shrimp, Miyakea nepa. Probiotics Antimicrob. Proteins 7, 275–283. https://doi.org/10.1007/s12602-015-9198-2 view
  65. Sruthy, K.S., Nair, A., Cubelio, S.S., Bright Singh, I.S., Philip, R., 2015b. Molecular characterization and phylogenetic analysis of an anti-lipopolysaccharide factor from the crucifix crab, Charybdis feriatus. Open Access Anim. Physiol. 149. https://doi.org/10.2147/OAAP.S84508 view
  66. Sudheer, N.S., Poulose, G., Thomas, A., Viswanath, K., Kulkarni, A., Narayanan, R.B., Philip, R., Singh, I.S.B., 2015. Expression profile of bio-defense genes in Penaeus monodon gills in response to formalin inactivated white spot syndrome virus vaccine. Antiviral Res. 117, 60–68. https://doi.org/10.1016/j.antiviral.2015.02.008 view
  67. Wilson, W., Lowman, D.W., Antony, S.P., Jayesh, P., Bright Singh, I.S., Philip, R., 2015. Immune gene expression profile of Penaeus monodon in response to marine yeast glucan application and white spot syndrome virus challenge. Fish Shellfish Immunol. 43, 346–356. https://doi.org/10.1016/j.fsi.2014.12.032 view
  68. Jose, D., Jayesh, P., Gopinath, P., Mohandas, A., Bright Singh, I.S., 2014. Potential application of beta-1, 3 glucanase from an environmental isolate of Pseudomonas aeruginosa MCCB 123 in fungal DNA extraction. Indian J. Exp. Biol. 52, 89–96. view
  69. Joseph, V., Haseeb, M., Ranjit, S., Anas, A., Bright Singh, I.S., 2014. Shrimp Production under Zero Water Exchange Mode Coupled with Bioremediation and Application of Probiotics. J. Fish. Int. 9, 5–14. view
  70. Karthik, V., Kamalakannan, V., Thomas, A., Sudheer, N.S., Bright Singh, I.S., Narayanan, R.B., 2014. Functional Characterization of a c-type Lysozyme from Indian Shrimp Fenneropenaeus indicus. Probiotics Antimicrob. Proteins 6, 114–121. https://doi.org/10.1007/s12602-013-9146-y view
  71. Kulkarni, A.D., Kiron, V., Rombout, J.H.W.M., Brinchmann, M.F., Fernandes, J.M.O., Sudheer, N.S., Bright Singh, I.S., 2014. Protein profiling in the gut of Penaeus monodon gavaged with oral WSSV-vaccines and live white spot syndrome virus. Proteomics 14, 1660–1673. https://doi.org/10.1002/pmic.201300405 view
  72. Poulose, S., Bright Singh, I.S., 2014. Optimization of culture conditions for the production of single cell protein from marine yeast Candida MCCF 101 as feed supplement in aquaculture. J. Aquat. Biol. Fish. 2, 283–289. view
  73. Rejish Kumar, V.J., Joseph, V., Philip, R., Singh, I.S.B., 2014. Nitrification by Stringed Bed Suspended Bioreactors at Different Salinities and Substrate Concentrations. Indian J. Geo-Marine Sci. 43. view
  74. Surekhamol, I.S., Deepa, G.D., Somnath Pai, S., Sreelakshmi, B., Varghese, S., Bright Singh, I.S., 2014. Isolation and characterization of broad spectrum bacteriophages lytic to Vibrio harveyi from shrimp farms of Kerala, India. Lett. Appl. Microbiol. 58, 197–204. https://doi.org/10.1111/lam.12175 view
  75. Syamkumar, R., Rojith, G., Rajathy, S., Bright Singh, I.S., 2014. Phytotoxicity assessment of coir pith effluent generated during lignin recovery process. Res. J. Chem. Sci. 4, 17–21. view
  76. Thomas, A., Sudheer, N.S., Viswanathan, K., Kiron, V., Bright Singh, I.S., Narayanan, R.B., 2014. Immunogenicity and protective efficacy of a major White Spot Syndrome Virus (WSSV) envelope protein VP24 expressed in Escherichia coli against WSSV. J. Invertebr. Pathol. 123, 17–24. https://doi.org/10.1016/j.jip.2014.08.004 view
  77. Chaithanya, E.R., Philip, R., Sathyan, N., Anil Kumar, P.R., Antony, S.P., Sanjeevan, V.N., Bright Singh, I.S., 2013a. A Novel Isoform of the Hepatic Antimicrobial Peptide, Hepcidin (Hepc-CB1), from a Deep-Sea Fish, the Spinyjaw Greeneye Chlorophthalmus bicornis (Norman, 1939): Molecular Characterisation and Phylogeny. Probiotics Antimicrob. Proteins 5, 1–7. https://doi.org/10.1007/s12602-012-9120-0 view
  78. Chaithanya, E.R., Philip, R., Sathyan, N., Anil Kumar, P.R., Cubelio, S.S., Bright Singh, I.S., 2013b. Molecular Characterisation and Phylogenetic Analysis of a Novel Isoform of Hepatic Antimicrobial Peptide, Hepcidin (Zc-hepc1), from the Coral Fish Moorish idol, Zanclus cornutus (Linnaeus, 1758). Probiotics Antimicrob. Proteins 5, 187–194. https://doi.org/10.1007/s12602-013-9139-x view
  79. Jacob, J.C., Ramya, K.D., Bright Singh, I.S., Philip, R., 2013. Bathymetric and latitudinal shift in sedimentary parameters along the shelf sediments of Bay of Bengal: Influence on heterotrophic bacteria. Adv. Appl. Sci. Res. 4, 119–133. view
  80. Jayesh, P., Jose, S., Philip, R., Bright Singh, I.S., 2013. A novel medium for the development of in vitro cell culture system from Penaeus monodon. Cytotechnology 65, 307–322. https://doi.org/10.1007/s10616-012-9491-9 view
  81. Kulkarni, A.D., Rombout, J.H.W.M., Bright Singh, I.S., Sudheer, N.S., Vlak, J.M.M., Caipang, C.M.A., Brinchmann, M.F., Kiron, V., 2013. Truncated VP28 as oral vaccine candidate against WSSV infection in shrimp: An uptake and processing study in the midgut of Penaeus monodon. Fish Shellfish Immunol. 34, 159–166. https://doi.org/10.1016/j.fsi.2012.10.028 view
  82. Kutty, S.N., Lawman, D., Bright Singh, I.S., Philip, R., 2013. Black yeasts from the slope sediments of Bay of Bengal : phylogenetic and functional characterization. Mycosphere 4, 346–361. view
  83. Manjusha, K., Jayesh, P., Jose, D., Sreelakshmi, B., Priyaja, P., Gopinath, P., Saramma, A. V., Bright Singh, I.S., 2013. Alkaline protease from a non-toxigenic mangrove isolate of Vibrio sp. V26 with potential application in animal cell culture. Cytotechnology 65, 199–212. https://doi.org/10.1007/s10616-012-9472-z view
  84. Ramya, K.D., Jacob, J.C., Correya, N.S., Bright Singh, I.S., Philip, R.,  2013. Biogeochemistry of the shelf sediments of south eastern Arabian sea: Effect on benthic bacterial heterotrophs. Adv. Appl. Sci. Res. 4, 315–328. view
  85. Rejish Kumar, V.J., Sukumaran, V., Achuthan, C., Joseph, V., Philip, R., Bright Singh, I.S., 2013. Molecular characterization of the nitrifying bacterial consortia employed for the activation of bioreactors used in brackish and marine aquaculture systems. Int. Biodeterior. Biodegradation 78, 74–81. https://doi.org/10.1016/j.ibiod.2013.01.002 view
  86. Rojith, G., Bright Singh, I.S., 2013a. Hydrogen peroxide pretreatment efficiency comparison and characterization of lignin recovered from coir pith black liquor. J. Environ. Res. Dev. 7, 1333–1339. view
  87. Rojith, G., Bright Singh, I.S., 2013b. Cellulose crystallanity change assessment of Biochar produced by pyrolysis of Coir pith. Res. J. Recent Sci. 2, 1–6. view
  88. Sathyan, N., Philip, R., Chaithanya, E.R., Anil Kumar, P.R., Sanjeevan, V.N., Singh, I.S.B., 2013. Characterization of Histone H2A Derived Antimicrobial Peptides, Harriottins, from Sicklefin Chimaera Neoharriotta pinnata (Schnakenbeck, 1931) and Its Evolutionary Divergence with respect to CO1 and Histone H2A. ISRN Mol. Biol. 2013, 1–10. https://doi.org/10.1155/2013/930216 view
  89. Sreedharan, K., Philip, R., Singh, I.S.B., 2013. Characterization and virulence potential of phenotypically diverse Aeromonas veronii isolates recovered from moribund freshwater ornamental fishes of Kerala, India. Antonie Van Leeuwenhoek 103, 53–67. https://doi.org/10.1007/s10482-012-9786-z view
  90. Afsal, V. V., Antony, S.P., Chaithanya, E.R., Bright Singh, I.S., Philip, R., 2012a. Two isoforms of anti-lipopolysaccharide factors identified and characterized from the hemocytes of portunid crabs, Portunus pelagicus and Scylla tranquebarica. Mol. Immunol. 52, 258–263. https://doi.org/10.1016/j.molimm.2012.06.003 view
  91. Afsal, V. V., Antony, S.P., Sanjeevan, V.N., Anil Kumar, P.R., Bright Singh, I.S., Philip, R., 2012b. A new isoform of anti-lipopolysaccharide factor identified from the blue swimmer crab, Portunus pelagicus: Molecular characteristics and phylogeny. Aquaculture 356–357, 119–122. https://doi.org/10.1016/j.aquaculture.2012.05.028 view
  92. Asok, A., Arshad, E., Jasmin, C., Somnath Pai, S., Bright Singh, I.S., Mohandas, A., Anas, A., 2012. Reducing Vibrio load in Artemia nauplii using antimicrobial photodynamic therapy: a promising strategy to reduce antibiotic application in shrimp larviculture. Microb. Biotechnol. 5, 59–68. https://doi.org/10.1111/j.1751-7915.2011.00297.x view
  93. Jayesh, P., Seena, J., Bright Singh, I.S., 2012. Establishment of Shrimp Cell Lines: Perception and Orientation. Indian J. Virol. 23, 244–251. https://doi.org/10.1007/s13337-012-0089-9 view
  94. Jose, S., Jayesh, P., Sudheer, N.S., Poulose, G., Mohandas, A., Philip, R., Bright Singh, I.S., 2012. Lymphoid organ cell culture system from Penaeus monodon (Fabricius) as a platform for white spot syndrome virus and shrimp immune-related gene expression. J. Fish Dis. 35, 321–334. https://doi.org/10.1111/j.1365-2761.2012.01348.x view
  95. Sathyan, N., Philip, R., Chaithanya, E.R., Anil Kumar, P.R., 2012. Identification and Molecular Characterization of Molluskin, a Histone-H2A-Derived Antimicrobial Peptide from Molluscs. ISRN Mol. Biol. 2012, 1–6. https://doi.org/10.5402/2012/219656 view
  96. Smitha, S., Haseena, V.S., Narayanan, T.N., Reena Mary, A.P., Ajayan, P.M., Puthumana, J., Aziz, A.A., Aburto, R.R., Mani, S.A., Joy, P.A., Anantharaman, M.R., 2012. Fluorescent Superparamagnetic Iron Oxide Core–Shell Nanoprobes for Multimodal Cellular Imaging. Mater. Express 2, 265–274. https://doi.org/10.1166/mex.2012.1083 view
  97. Sreedharan, K., Philip, R., Singh, I.S.B., 2012. Virulence potential and antibiotic susceptibility pattern of motile aeromonads associated with freshwater ornamental fish culture systems: a possible threat to public health. Brazilian J. Microbiol. 43, 754–765. https://doi.org/10.1590/S1517-83822012000200040 view
  98. Sudheer, N.S., Philip, R., Bright Singh, I.S., 2012. Anti-white spot syndrome virus activity of Ceriops tagal aqueous extract in giant tiger shrimp Penaeus monodon. Arch. Virol. 157, 1665–1675. https://doi.org/10.1007/s00705-012-1346-3 view
  99. Antony, S.P., Bright Singh, I.S., Jose, R.M., Anil Kumar, P.R., Philip, R., 2011a. Antimicrobial peptide gene expression in tiger shrimp, Penaeus monodon in response to gram-positive bacterial probionts and white spot virus challenge. Aquaculture 316, 6–12. https://doi.org/10.1016/j.aquaculture.2011.03.025 view
  100. Antony, S.P., Bright Singh, I.S., Philip, R., 2011b. Molecular characterization and phylogenetic analysis of a penaeidin-like antimicrobial peptide, Fi-penaeidin from Fenneropenaeus indicus. Aquaculture 319, 298–301. https://doi.org/10.1016/j.aquaculture.2011.06.011 view
  101. Antony, S.P., Bright Singh, I.S., Sudheer, N.S., Vrinda, S., Priyaja, P., Philip, R., 2011c. Molecular characterization of a crustin-like antimicrobial peptide in the giant tiger shrimp, Penaeus monodon, and its expression profile in response to various immunostimulants and challenge with WSSV. Immunobiology 216, 184–194. https://doi.org/10.1016/j.imbio.2010.05.030 view
  102. Antony, S.P., Philip, R., Joseph, V., Bright Singh, I.S., 2011d. Anti-lipopolysaccharide factor and crustin-III, the anti-white spot virus peptides in Penaeus monodon: Control of viral infection by up-regulation. Aquaculture 319, 11–17. https://doi.org/10.1016/j.aquaculture.2011.06.022 view
  103. Jose, S., Jayesh, P., Mohandas, A., Philip, R., Bright Singh, I.S., 2011. Application of primary haemocyte culture of Penaeus monodon in the assessment of cytotoxicity and genotoxicity of heavy metals and pesticides. Mar. Environ. Res. 71, 169–177. https://doi.org/10.1016/j.marenvres.2010.12.008 view
  104. Kumar, V.J.R., Joseph, V., Vijai, R., Philip, R., Singh, I.S.B., 2011. Nitrification in a packed bed bioreactor integrated into a marine recirculating maturation system under different substrate concentrations and flow rates. J. Chem. Technol. Biotechnol. 86, 790–797. https://doi.org/10.1002/jctb.2588 view
  105. Sreedharan, K., Rosamma, P., Bright Singh, I.S., 2011. Isolation and characterization of virulent Aeromonas veronii from ascitic fluid of oscar Astronotus ocellatus showing signs of infectious dropsy. Dis. Aquat. Organ. 94, 29–39. https://doi.org/10.3354/dao02304 view
  106. Sudheer, N.S., Philip, R., Bright Singh, I.S., 2011. In vivo screening of mangrove plants for anti WSSV activity in Penaeus monodon, and evaluation of Ceriops tagal as a potential source of antiviral molecules. Aquaculture 311, 36–41. https://doi.org/10.1016/j.aquaculture.2010.11.016 view
  107. Antony, S.P., Bright Singh, I.S., Philip, R., 2010. Molecular characterization of a crustin-like, putative antimicrobial peptide, Fi-crustin, from the Indian white shrimp, Fenneropenaeus indicus. Fish Shellfish Immunol. 28, 216–220. https://doi.org/10.1016/j.fsi.2009.10.013 view
  108. Jose, S., Mohandas, A., Philip, R., Bright Singh, I.S., 2010. Primary hemocyte culture of Penaeus monodon as an in vitro model for white spot syndrome virus titration, viral and immune related gene expression and cytotoxicity assays. J. Invertebr. Pathol. 105, 312–321. https://doi.org/10.1016/j.jip.2010.08.006 view
  109. Rejish Kumar, V.J., Joseph, V., Philip, R., Bright Singh, I.S., 2010. Nitrification in brackish water recirculating aquaculture system integrated with activated packed bed bioreactor. Water Sci. Technol. 61, 797. https://doi.org/10.2166/wst.2010.849 view
  110. Anas, A., Lowman, D.W., Williams, D.L., Millen, S., Somnath Pai, S., Sajeevan, T.P., Philip, R., Bright Singh, I.S., 2009. Alkali insoluble glucan extracted from Acremonium diospyri is a more potent immunostimulant in the Indian White Shrimp, Fenneropenaeus indicus than alkali soluble glucan. Aquac. Res. 40, 1320–1327. https://doi.org/10.1111/j.1365-2109.2009.02231.x view
  111. Manju, N.J., Deepesh, V., Achuthan, C., Philip, R., Bright Singh, I.S., 2009. Immobilization of nitrifying bacterial consortia on wood particles for bioaugmenting nitrification in shrimp culture systems. Aquaculture 294, 65–75. https://doi.org/10.1016/j.aquaculture.2009.05.008 view
  112. Manjusha, M., Varghese, R., Philip, R., Mohandas, A., Bright Singh, I.S., 2009. Pathological changes in Fenneropenaeus indicus experimentally infected with white spot virus and virus morphogenesis. J. Invertebr. Pathol. 102, 225–232. https://doi.org/10.1016/j.jip.2009.08.011 view
  113. Preetha, R., Jose, S., Prathapan, S., Vijayan, K.K., Jayaprakash, N.S., Philip, R., Bright Singh, I.S., 2009. An inhibitory compound produced by Pseudomonas with effectiveness on Vibrio harveyi. Aquac. Res. 41, 1452–1461. https://doi.org/10.1111/j.1365-2109.2009.02436.x view
  114. Rejish Kumar, V.J., Achuthan, C., Manju, N.J., Philip, R., Bright Singh, I.S., 2009a. Mass production of nitrifying bacterial consortia for the rapid establishment of nitrification in saline recirculating aquaculture systems. World J. Microbiol. Biotechnol. 25, 407–414. https://doi.org/10.1007/s11274-008-9905-1 view
  115. Rejish Kumar, V.J., Achuthan, C., Manju, N.J., Philip, R., Bright Singh, I.S., 2009b. Activated packed bed bioreactor for rapid nitrification in brackish water hatchery systems. J. Ind. Microbiol. Biotechnol. 36, 355–365. https://doi.org/10.1007/s10295-008-0504-9 view
  116. Rejish Kumar, V.J., Achuthan, C., Manju, N.J., Philip, R., Bright Singh, I.S., 2009c. Stringed bed suspended bioreactors (SBSBR) for in situ nitrification in penaeid and non-penaeid hatchery systems. Aquac. Int. 17, 479–489. https://doi.org/10.1007/s10499-008-9218-2 view
  117. Sajeevan, T. P., Philip, R., Bright Singh, I.S., 2009. Dose/frequency: A critical factor in the administration of glucan as immunostimulant to Indian white shrimp Fenneropenaeus indicus. Aquaculture 287, 248–252. https://doi.org/10.1016/j.aquaculture.2008.10.045 view
  118. Somnath Pai, S., Anas, A., Jayaprakash, N.S., Priyaja, P., Sreelakshmi, B., Preetha, R., Philip, R., Mohandas, A., Bright Singh, I.S., 2009a. Penaeus monodon larvae can be protected from Vibrio harveyi infection by pre-emptive treatment of a rearing system with antagonistic or non-antagonistic bacterial probiotics. Aquac. Res. 41, 847–860. https://doi.org/10.1111/j.1365-2109.2009.02362.x view
  119. Somnath Pai, S., Manjusha, M., Anas, A., Ranjit, S., Sudheer, N.S., Philip, R., Bright Singh, I.S., 2009b. A survey of potential carriers and reservoir of White Spot Syndrome Virus in Penaeus monodon culture systems of Kerala. Indian J. Virol. 20. view
  120. Anas, A., Philip, R., Bright Singh, I.S., 2008. Chitosan as a wall material for a microencapsulated delivery system for Macrobrachium rosenbergii (de Man) larvae. Aquac. Res. 39, 885–890. https://doi.org/10.1111/j.1365-2109.2008.01944.x view
  121. Preetha, R., Jayaprakash, N.S., Bright Singh, I.S., 2007a. Synechocystis MCCB 114 and 115 as putative probionts for Penaeus monodon post-larvae. Dis. Aquat. Organ. 74, 243–247. https://doi.org/10.3354/dao074243 view
  122. Preetha, R., Jayaprakash, N.S., Philip, R., Bright Singh, I.S., 2007b. Optimization of carbon and nitrogen sources and growth factors for the production of an aquaculture probiotic (Pseudomonas MCCB 103) using response surface methodology. J. Appl. Microbiol. 102, 1043–1051. https://doi.org/10.1111/j.1365-2672.2006.03149.x view
  123. Preetha, R., Jayaprakash, N.S., Philip, R., Bright Singh, I.S., 2007c. Optimization of medium for the production of a novel aquaculture probiotic,Micrococcus MCCB 104 using central composite design. Biotechnol. Bioprocess Eng. 12, 548–555. https://doi.org/10.1007/BF02931353 view
  124. Achuthan, C., Rejish Kumar, V.J., Manju, N.J., Philip, R., Bright Singh, I.S., 2006. Development of nitrifying bacterial consortia for immobilizing in nitrifying bioreactors designed for penaeid and non-penaeid larval rearing systems in the tropics. Indian J. Mar. Sci. 35, 240–248. view
  125. Amar, B., Philip, R., Bright Singh, I.S., 2006. Efficacy of fermented prawn shell waste as a feed ingredient for Indian white prawn, Fenneropenaeus indicus. Aquac. Nutr. 12, 433–442. https://doi.org/10.1111/j.1365-2095.2006.00445.x view
  126. Jayaprakash, N.S., Rejish Kumar, V.J., Philip, R., Bright Singh, I.S., 2006a. Vibrios associated with Macrobrachium rosenbergii (De Man, 1879) larvae from three hatcheries on the Indian southwest coast. Aquac. Res. 37, 351–358. https://doi.org/10.1111/j.1365-2109.2006.01432.x view
  127. Jayaprakash, N.S., Somnath Pai, S., Philip, R., Bright Singh, I.S., 2006b. Isolation of a pathogenic strain of Vibrio alginolyticus from necrotic larvae of Macrobrachium rosenbergii (de Man). J. Fish Dis. 29, 187–191. https://doi.org/10.1111/j.1365-2761.2006.00690.x view
  128. Mani, S.K., Kanjur, R., Bright Singh, I.S., Reed, R.H., 2006. Comparative effectiveness of solar disinfection using small-scale batch reactors with reflective, absorptive and transmissive rear surfaces. Water Res. 40, 721–727. https://doi.org/10.1016/j.watres.2005.11.039 view
  129. Sahul Hameed, A.S.S., Parameswaran, V., Shukla, R., Bright Singh, I.S., Thirunavukkarasu, A.R.R., Bhonde, R.R.R., 2006. Establishment and characterization of India’s first marine fish cell line (SISK) from the kidney of sea bass (Lates calcarifer). Aquaculture 257, 92–103. https://doi.org/10.1016/j.aquaculture.2006.01.011 view
  130. Sajeevan, T.P., Philip, R., Bright Singh, I.S., 2006. Immunostimulatory effect of a marine yeast Candida sake S165 in Fenneropenaeus indicus. Aquaculture 257, 150–155. https://doi.org/10.1016/j.aquaculture.2006.03.008 view
  131. Vijayan, K.K., Bright Singh, I.S., Jayaprakash, N.S., Alavandi, S. V., Somnath Pai, S., Preetha, R., Rajan, J.J.S., Santiago, T.C., 2006. A brackishwater isolate of Pseudomonas PS-102, a potential antagonistic bacterium against pathogenic vibrios in penaeid and non-penaeid rearing systems. Aquaculture 251, 192–200. https://doi.org/10.1016/j.aquaculture.2005.10.010 view
  132. Anas, A., Paul, S., Jayaprakash, N.S., Philip, R., Bright Singh, I.S., 2005. Antimicrobial activity of chitosan against vibrios from freshwater prawn Macrobrachium rosenbergii larval rearing systems. Dis. Aquat. Organ. 67, 177–179. https://doi.org/10.3354/dao067177 view
  133. Bright Singh, I.S., Manjusha, M., Somnath Pai, S., Philip, R., Bright Singh, I.S., 2005. Fenneropenaeus indicus is protected from white spot disease by oral administration of inactivated white spot syndrome virus. Dis. Aquat. Organ. 66, 265–270. https://doi.org/10.3354/dao066265 view
  134. Jayaprakash, N.S., Somnath Pai, S., Anas, A., Preetha, R., Philip, R., Bright Singh, I.S., 2005. A marine bacterium, Micrococcus MCCB 104, antagonistic to vibrios in prawn larval rearing systems. Dis. Aquat. Organ. 68, 39–45. https://doi.org/10.3354/dao068039 view
  135. Ajitha, S., Sridhar, M., Bright Singh, I.S., Varghese, V., 2004. Probiotic Effects of Lactic Acid Bacteria gainst Vibrio Alginolyticus in Penaeus (Fenneropenaeus) indicus (H. Milne Edwards). Asian Fish. Sci. 17, 71–80 view
  136. Ramachandran, K., Bright Singh, I.S., 2004. Optimum growth requirements of nitrifying consortia developed from treated sewage. Indian J. Exp. Biol. 42, 314–318. view
  137. Anas, A., Bright Singh, I.S., 2003. Application of yeast glucan and bacterins for enhanced seed production of Macrobrachium rosenbergii (de Man). J. Aquac. Trop. 18. view
  138. Nambudiri, D.D., George, S., Bright Singh, I.S., Sherief, P.M., 2001. Influence of storage conditions on the survival period of the clam Meretrix casta. J. Food Sci. Technol. 38, 379–380.
  139. Sunil Kumar, G., Bright Singh, I.S., Philip, R., 2001. Development of a cell culture system from the ovarian tissue of African catfish (Clarias gariepinus). Aquaculture 194, 51–62. https://doi.org/10.1016/S0044-8486(00)00509-3 view
  140. Philip, R., Sunil Kumar, G., Bright Singh, I.S., 2000. Development of cell culture system from liver, kidney, and spleen of African catfish, Clarias gariepinus. Vitr. – Cell. Dev. Biol. 36, 84.
  141. Vici, V., Bright Singh, I.S., Bhat, S.G., 2000. Application of bacterins and yeast Acremonium dyosporii to protect the larvae of Macrobrachium rosenbergii from vibriosis. Fish Shellfish Immunol. 10, 559–563. https://doi.org/10.1006/fsim.2000.0278 view
  142. Bright Singh, I.S., Lakshmanaperumalsamy, P., Chandramohan, D., 1998. Bacterial flora of pond reared Penaeus indicus (Milne Edwards). J. Aquac. Trop. 13, 133–142. [view]
  143. Bright Singh, I.S., Lakshmanaperumalsamy, P., Chandramohan, D., 1981. Finrot diseases in Etroplus suratensis. Bull. Dep. Mar. Sci. Univ. Cochin 12, 147–154.
  144. Bright Singh, I.S., Lakshmanaperumalsamy, P., Thomas, I., Chandrasekharan, M., Chandramohan, D., 1982. Bacteria associated with brown spot disease in Penaeus indicus H. Milne Edwards. Bull. Dep. Mar. Sci. Univ. Cochin.
  145. Bright Singh, I.S., Philip, R., 1995. A simple device for the separation of weak larvae of Macrobrachium rosenbergii (De Man). Aquac. Res. 26, 225–227. https://doi.org/10.1111/j.1365-2109.1995.tb00906.x view
  146. Bright Singh, I.S., Philip, R., 1993. Comparative efficiency of three media in isolating heterotrophic bacteria from the larvae and larval rearing water of Macrobrachium rosenbergii. Indian J. Microbiol. 33, 67–69.
  147. Bright Singh, I.S., Philip, R., Maqbool, T.K., Ramesh, S., 1994. Bacteria associated with Epizootic Ulcerative Syndrome in fishes on inland waters of Kerala. Indian J. Microbiol. 34, 233. 
  148. Bright Singh, I.S., Philip, R., Raveendranath, M., Shanmugam, J., 1995. Development of primary cell cultures from kidney of freshwater fish Heteropneustes fossilis. Indian J. Exp. Biol. 33, 595–599.
  149. Lakshmanaperumalsamy, P., Chandrasekaran, P.M., Bright Singh, I.S., Chandramohan, D., 1981. Microbial indicators and pathogens near the mouth region of Vembanad Lake. Bull. Dep. Mar. Sci. Univ. Cochin 12, 103–109.
  150. Nambudiri, D.D., Bright Singh, I.S., George, S., Sherief, P.M., Maniappan, C., Ayoob, T.M., 1995. Design, fabrication and standardisation of a depuration system for bivalves. Fish. Technol. 32., 126-130. view
  151. Sen, A., Bright Singh, I.S., Rengarajan, R., Philip, R., Kumar, G.S., Sen, A., 1999. Evidence of a Bacilliform Virus Causing Outbreaks of Whitespot Disease in Penaeus monodon H. Milne Edwards in India. Asian Fish. Sci. 12, 41–47. view
  152. Sunil Kumar, G., Bright Singh, I.S., Philip, R., Raveendranath, M., Shanmugam, J., 1998. Efficacy of fish and prawn muscle extracts as supplements of medium in development of a primary cell culture system from larval tissue of aquarium fish Poecilia reticulata. Indian J. Exp. Biol. 36, 91–94.
  1. Anoop B.S. (Reg. No. 4846) Establishment of Shrimp- Insect Hybrid Cell Line PmLyO –Sf 9 and Development of Transduction Based Oncogenic Induction for Shrimp Cell Immortalisation.
  2. Sowmya P.Mohandas (Reg. No.  5954) Development of nanoparticles and films of polyhydroxyalkanoates from marine bacteria 29-6-2020
  3. Boobal R. (Reg. No. 4956) Dynamics of microbial population in packed bed nitrifying bioreactor integrated with shrimp maturation system under RAS mode 14-10-202
  4. Deepa G.D. (Reg. No. 3474) Putative probiotic Cyanobacterium spp. from the coastal waters of Cochin: Polyphasic taxonomy, fate of axenic cultures and heterotrophic bacterial associates 22-10-2019
  5. Ammu Thomas (Reg. No. 3471) Photosynthetic bacteria for bioremediation of hydrogen sulphide in aquatic systems 30-5-2017
  6. Preena P.G. (Reg. No. 3621) Molecular basis of community composition and function of nitrifying – denitrifying bacterial consortia used in bio-augmenting nitrification in aquaculture systems 5-6-2016
  7. Asha P. (Reg. No. 3960) Novel cellulases through culture – dependent and metagenomics based approaches: Discovery and characterization 1-10-2016
  8. Sareen Saraha John (Reg. No. 3473) Bioprocess optimization and characterization of phytase from an environmental isolate Bacillus MCCB 242 18-1-2016
  9. Rojith, (Reg. No. 3573) Delignification of coir pith and development of products having agro – industrial applications 18-2-2016
  10. Deepa S. Nair (Reg. No. 3569) Polyhydroxyalkanoates from Vibrio sp. MCCB 237: Isolation of the organism, medium optimization, polymer extraction and characterization 27-6-2017
  11. Riya George (Reg. No. 3570) Bacillus cereus MCCB 101 as bioaugmentor for detritus degradation in simulated zero water exchange shrimp grow out system 20-5-2015
  12. Deepesh, V. (Reg. No. 2905) Development and validation of bacterial consortia for bioremediation of detritus in aquaculture systems. 15-11-2014
  13. Prem Gopinath (Reg. No.3235) Mosquito larvae borne viruses from the central Travancore region of Kerala: isolation and characterization 9-3-2015
  14. Rose Mary Jose (Reg. No. 3234) Efficacy of marine bacterial probiotics on shrimp post larvae for enhanced growth, survival and protection from Vibrio harveyi” 10-9-2015
  15. Sunitha Poulose (Reg. No. 32.33) Marine yeast Candida MCCF 101 as feed supplement in aquaculture: nutritional quality, optimization of large scale production and evaluation of its protective effect on koi carp from Aeromonas infection Development of CHH family recombinant hormones and RNAi for induced maturation of Penaeus monodon 20-10-2014
  16. Vrinda, S (Reg. 3113) Marine yeast Candida MCCF 101 as feed supplement in aquaculture: nutritional quality, optimization of large scale production and evaluation of its protective effect on koi carp from Aeromonas infection Development of CHH family recombinant hormones and RNAi for induced maturation of Penaeus monodon 8-4-2014
  17. Suneesh, K.S. (Reg. No. 2652) A marine actinomycete Nocardiopsis MCCB 110 as source of novel drugs to manage vibriosis 12-12-2013
  18. Ranjit, K. (Reg. No. 2600) Solar photo – oxidative disinfection for generating potable water from Natural sources 21-12-2013
  19. Haseeb M (Reg. No.3232) Development of zero water exchange shrimp culture systems integrated with bioremediation of detritus and ammonia –nitrogen 15-11-2013
  20. Surekha mol, I.S (Reg. No.3072) Vibrio harveyi phages: Isolation, characterization and evaluation of their potential as phage therapeutics on Vibrio harveyi in shrimp hatcheries 17-8-2013
  21. Jayesh P (Reg. No.3469) Development of lymphoid cell culture system from Penaeus monodon and molecular approaches for its transformation 9 – 5- 2013
  22. Priyaja P (Reg. no. 2877) Pyocyanin (5-Methyl – 1- hydroxyphenazine) produced by Pseudomonas aeruginosa as antagonist to Vibrios in aquaculture: Over expression, downstream process and toxicity 28-12-2012
  23. Divya Jose (Reg. No.3065) Proteases from an environmental isolate of Pseudomonas aeruginosa MCCB 123 and their applications 3 -7- 2012
  24. B. Sreelakshmi (Reg. no. 3071) Molecular approaches for characterization of pathogenicity of vibrios with special reference to Vibrio harveyi from Penaeus monodon larval production system 26 – 4 – 2013
  25. Gigi Paulose (Reg. no.2655) Immune response of Penaeus monodon to the inactivated white spot syndrome virus preparation 7- 2-2012
  26. Seena Jose (Reg. no: 2651) Cell culture system from Penaeus monodon: Development and application 28-10- 2010
  27. Sudheer N.S (Reg. no. 2762) Mangrove plants Ceriops tagal as a potential source of anti White Spot Syndrome Virus preparation for Penaeus monodon 30 -12- 2010
  28. Sreedharan. K (Reg. no. 2502) Aeromonas associated with freshwater ornamental fish culture systems: Characterization, pathogenicity and management. 20-1- 2010
  29. Rejish Kumar V.J (Reg. no. 2480) Nitrifying bioreactors integrated in to shrimp and prawn hatchery systems: Molecular characterization of the nitrifying bacterial consortia, reactor kinetics, modeling and validation 22-3- 2009
  30. Manju, N.J (Reg. no. 2432) Development of nitrifying and photosynthetic sulphur bacteria based bioaugmentation systems for the bioremediation of ammonia and hydrogen sulphide in shrimp culture 21-8- 2008
  31. Somnath Pai (Reg. no. 2362) Biocontrol of Vibrio harveyi in Penaeus monodon larval rearing systems employing probiotics and vibriophages. 15-10- 2007
  32. R. Preetha (Reg. no.2360) Bioprocess technology for antagonistic Pseudomonas MCCB 102 and 103, Micrococcus MCCB 104, and evaluation of Synechocystis MCCB 114, 115 as probiotics for the management of Vibrio in shrimp culture systems 28-7- 2007
  33. A. Anas (Reg. no.2361) Extraction of glucan from Acremonium diospyri and its application in Macrobrachium rosenbergii larval rearing system along with bacterins as microspheres 26-8- 2006
  34. N. S. Jayaprakash Development of microbial technology for exclusion of vibrios from prawn larval rearing systems. 27-5- 2006
  35. M. Manjusha (Reg. no. 1953) White spot disease in penaeids: Development of immunodiagnostics and vaccine. 15-6- 2004
  36. G. Sunil Kumar Development of cell culture systems from selected species of fish and prawns. 8- 6- 2001
  37. Cini Achuthan (Reg. no. 1583) Development of bioreactors for nitrifying water in closed system hatcheries of penaeid and non-penaeid prawns. 18- 11- 2000
  38. P. I. Narayanan Development of microbial treatment of ret liquor generated in a coir retting bioreactor. 2-5-1999
  39. T.R.Sathyakeerthy Polyphenolic compounds liberated during coir retting in a bioreactor: Separation, characterization and possible applications. 15 -9- 1999
  40. Sarita G. Bhat (Reg. no.1431) Systematics and pathogenicity of Vibrionaceae associated with the larvae of Macrobrachium rosenbergii in hatchery 24 -10- 1998
  41. Kavitha Ramachandran Development of bioreactors for nitrifying sewage 19-9-1998
  • Coir Retting Bioreactor – Patent Application No. 130/MA/2002 dated 18/02/2002. Patent No.198886 dated: 18.2.2002 (Inventors: Prof. I.S. Bright Singh; Dr. T.R. Satyakeerthy; Dr. P.I. Narayanan; Dr. N.S.Jayaprakash and Dr. A. Mohandas). Assignee: School of Environmental Studies, Cochin University of Science and Technology and Coir Board, Government of India.
  • Bioreactors for nitrifying water in closed system hatcheries of penaeid and non-penaeid prawns – Patent Application No. : 828/DEL/2000 dated 13.9.2000. Patent no. 241648. (Inventors: Prof. I.S.Bright Singh; Dr. Cini Achuthan and Dr. Rosamma Philip). School of Environmental Studies, Cochin University of Science and Technology and Department of Biotechnology, Government of India
  • Depuration System – Patent file No. 75/MAS/95. (Inventors: Prof. D. D. Nambudiri; Prof. I.S. Bright Singh; Prof. Sajan George and Prof. P. M. Sherief). Assignee: College of Fisheries, Kerala Agricultural University and Indian Council of Agricultural Research).
  • Process of extracting anti – white spot syndrome virus molecules from mangrove plants – Patent application # 2607/DEL/2006 dated 5.12.2006. Patent no. 254984. ( Inventors: Dr. N.S. Sudheer; Dr. Rosamma Philip and Prof. I.S. Bright Singh). Assignee: National Centre for Aquatic Animal Health, Cochin University of Science and Technology and Department of Biotechnology, Government of India.
  • Bioprocess Technology for the mass production of an aquaculture probiotic Pseudomonas MCCB 102. Patent application no. 1714.CHE/2009 dated 20.7.1009.( Inventors: Dr. R. Preetha; Dr. K.K.Vijayan; Dr. Rosamma Philip; Prof. I.S.Bright Singh). Assignee: National Centre for Aquatic Animal Health, Cochin University of Science and Technology and Ministry of Earth Science, Government of India.
  • Bioprocess technology for the mass production of an aquaculture probiotic Micrococcus MCCB 104 – Regd# IPR/4.9.2.1/10030. (Inventors: Prof. I.S. Bright Singh; Dr. R. Preetha; Dr. N.S. Jayaprakash; Dr. Rosamma Philip). Assignee: National Centre for Aquatic Animal Health, Cochin University of Science and Technology and Ministry of Earth Science, Government of India.
  • Bioprocess technology for the mass production of an aquaculture probiotic Pseudomonas MCCB 103. Regd. # IPR/4.9.2.1/10030. (Inventors: Prof. I.S. Bright Singh; Dr. R. Preetha; Dr. N.S. Jayaprakash; Dr. Rosamma Philip). Assignee: National Centre for Aquatic Animal Health, Cochin University of Science and Technology and Ministry of Earth Science.
  • Development of a novel cell culture medium SCCM (Shrimp Cell Culture Medium) exclusively for shrimp cell culture. Patent application submitted to DBT on 15.2.2012. (Inventors: Prof. I. S. Bright Singh; Dr. Rosamma Philip; Dr. Jayesh, P and Dr. Seena Jose). Assignee: National Centre for Aquatic Animal Health, Cochin University of Science and Technology and Department of Biotechnology, Government of India.

National / International Conferences – 69 Nos

  • Best Thesis Award-Sareen sarah John- for thesis entitled ‘Bioprocess optimization and characterization of phytase from an environmental isolate Bacillus MCCB 242’ guided by Dr.I.S.Bright Singh by Society for Educational & Scientific Research, Mumbai during the International conference on Green Technology for Health and Environment held at Bangalore during 15-16th December 2016.
  • Best Thesis Award -Sunitha Poulose- for thesis entitled “Marine yeast Candida MCCF 101 as feed supplement in aquaculture: nutritional quality, optimization of large scale production and evaluation of its protective effect on Koi carp from Aeromonas infection” under the Guidance of Dr. I. S. Bright Singh on First International conference on “BIOSCIENCES: STATE OF THE ART ADVANCEMENT” organized by Society for Educational & Scientific Research (SESR).
  • Best Oral Paper Presentation Award- Sareen Sarah John and I. S. Bright Singh. “Improvisation of Phytase Production through Optimization of fermentation conditions using Central Composite Design of Response Surface Methodology” –– Proceedings of International Conference on Biosciences: State-Of-The- Art Advancements, Section: Enzyme and Metabolic Sciences, Kumarakom, Kottayam, 2014, P.51 (ISBN:818805880-7) (Best Paper award).
  • Best Oral Paper Presentation Award- P. Asha and I.S. Bright Singh , School of Environmental studies , Cochin presented paper entitled “Cellulase – Is pure culture study sufficient for cellulose saccharification?”(Section –Novel Research Methodologies in Biosciences).International conference on Biosciences organized by SESR.
  • Best Oral Paper Presentation Award-Riya George and I.S.Bright Singh. An evaluation of bioaugmentation efficacy of Bacillus cereus MCCB 101 shrimp pond sediment microcosms” . International Conference, “BIOSCIENCES: STATE-OF-THE-ARTADVANCEMENTS. Kumarakom, Kottayam,2014
  • Best Oral Presentation Award- P.G Preena and Bright Singh. 2013. Aerobic denitrifying potential of Nitrifying Bacterial consortia for activating nitrifying bioreactors in recirculating aquaculture systems. Emerging Trends in Indian Aquaculture.National Conference, Department Of Aquaculture, Karyavatom,Trivandrum,2013.
  • Best Oral Paper Presentation Award- Sunitha Poulose & Singh I.S.B. Feed supplementation with marine yeast Candida MCCF 101 enhances fish health: assessed through haematological parameters. National Conference, Department Of Aquaculture, Karyavatom,Trivandrum, 2013.
  • Best Oral Paper Presentation Award- Anoop. B.S and I.S.Bright Singh Transduction mediated expression of oncogene (H-ras) into primary lymphoid cell culture of Penaeus monodon for developing a continuous cell line.In: proceedings:International conference on biotechnology and its application in aquaculture and fisheries. CAS, Annamalai University, 2017
  1. Reed RH, Singh ISB, Mani SK, Kanjur R, Simon N, Mol S (2005) Making the most of a scarce resource – solar disinfection of drinking water in the arid zone of western Rajasthan. Arid Lands Newsletter. 57:May/June, http://cals.arizona.edu/OALS/ALN/aln57/reed.html

GenBank Submissions : 350

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