Journal Publications

  1. Anooja, V.V., Archana, K., Athira, P.P., Anju, M.V., Neelima, S., Muhammed Musthafa, S., Dhaneesha, M., Sajeevan, T.P., Singh, I.S.B., Philip, R., 2024. Antibacterial activity and modes of action of a novel hepcidin isoform from the shrimp scad, Alepes djedaba (Forsskål, 1775). Fish Shellfish Immunol. 146, 109406. https://doi.org/10.1016/j.fsi.2024.109406   

  2. Athira, P.P., Anju, M. V., Anooja, V. V., Archana, K., Neelima, S., Musthafa, S.M., Singh, I.S.B., Philip, R., 2024a. A Novel Beta-Defensin Isoform from Malabar Trevally, Carangoides malabaricus (Bloch & Schneider, 1801), an Arsenal Against Fish Bacterial Pathogens: Molecular Characterization, Recombinant Production, and Mechanism of Action. Mar. Biotechnol. https://doi.org/10.1007/s10126-024-10338-4

  3. Athira, P.P., Anooja, V. V., Anju, M. V, Archana, K., Neelima, S., Muhammed Musthafa, S., Bright Singh, I.S., Philip, R., 2024b. Antibacterial Efficacy and Mechanisms of Action of a Novel Beta-Defensin from Snakehead Murrel, Channa striata. Probiotics Antimicrob. Proteins. https://doi.org/10.1007/s12602-024-10307-2

  4. Diya Dominic, D.V., Muneer, A., Eldho, L., Jose, S., Sajeevan, T.P., Puthumana, J., Philip, R., Joseph, V., Bright Singh, I.S., 2024. Propagation of IHHNV (Infectious Hypodermal and Haematopoietic Necrosis Virus)/PstDVI and MBV (Monodon baculovirus)/PmNV in shrimp primary haemocyte culture. J. Invertebr. Pathol. 108062. https://doi.org/10.1016/j.jip.2024.108062

  5. Kachiprath, B., Gopi, J., Sarasan, M., Puthumana, J., Chaithanya, E.R., Philip, R., 2024. Metavirome mining from fjord sediments of Svalbard Archipelago. J. Soils Sediments. https://doi.org/10.1007/s11368-024-03809-7
  6. Kachiprath, B., Solomon, S., Gopi, J., Jayachandran, P.R., Thajudeen, J., Sarasan, M., Mohan, A.S., Puthumana, J., Chaithanya, E.R., Philip, R., 2024. Exploring bacterial diversity in Arctic fjord sediments: a 16S rRNA–based metabarcoding portrait. Brazilian J. Microbiol. 55, 499–513. https://doi.org/10.1007/s42770-023-01217-6 

  7. Krishnaswami, I., Sabu, S., Bright Singh, I.S., Joseph, V., 2024. Marine benthic diatom Psammodictyon panduriforme (MACC 35) as a potent producer of long chain hydrocarbons and fatty acids of biofuel significance. Algal Res. 103492. https://doi.org/10.1016/j.algal.2024.103492

  8. Puthumana, J., Chandrababu, A., Sarasan, M., Joseph, V., Singh, I.S.B., 2024. Genetic improvement in edible fish: status, constraints, and prospects on CRISPR-based genome engineering. 3 Biotech 14, 44. https://doi.org/10.1007/s13205-023-03891-7

  9. Ryu, B., Glukhov, E., Teixeira, T.R., Caffrey, C.R., Madiyan, S., Joseph, V., Avalon, N.E., Leber, C.A., Naman, C.B., Gerwick, W.H., 2024. The Kavaratamides: Discovery of Linear Lipodepsipeptides from the Marine Cyanobacterium Moorena bouillonii Using a Comparative Chemogeographic Analysis. J. Nat. Prod. https://doi.org/10.1021/acs.jnatprod.4c00242

  10. Wilson, W., Lowman, D., Puthumana, J., Kuriakose, R., Singh, I.S.B., Philip, R., 2024. Biocompatible melanin from the marine black yeast Hortaea werneckii R23 with antioxidant and photoprotection property. Brazilian J. Microbiol. https://doi.org/10.1007/s42770-024-01483-y
  1. Anju, M. V., Archana, K., Anooja, V. V., Athira, P.P., Neelima, S., Singh, I.S.B., Philip, R., 2023. A novel anti-lipopolysaccharide factor from blue swimmer crab Portunus pelagicus and its cytotoxic effect on the prokaryotic expression host, E. coli on heterologous expression. J. Genet. Eng. Biotechnol. 21, 22. https://doi.org/10.1186/s43141-023-00478-w
  2. Dominic, D.V.D., Sajeevan, T.P., Ameer, A., Muneer, A., Eldho, L., Anoop, B.S., Jayesh, P., Joseph, V., Philip, R., Singh, I.S.B., 2023. In vitro propagation of infectious hypodermal hematopoietic necrosis virus (Penaeus stylirostris penstyldensovirus) in PmLyO-Sf9 cells. Arch. Virol. 168, 211. https://doi.org/10.1007/s00705-023-05827-1
  3. James, G., Prasannan Geetha, P., Thavarool Puthiyedathu, S., Vattringal Jayadradhan, R.K., 2023. Applications of Actinobacteria in aquaculture: prospects and challenges. 3 Biotech 13, 42. https://doi.org/10.1007/s13205-023-03465-7
  4. Jifiriya, M.J., Preena, P.G., Rejish Kumar, V.J., Nair, A.J., Joseph, V., 2023. Role of archaea in aquaculture: prospects and challenges. Aquac. Int. https://doi.org/10.1007/s10499-023-01317-y
  5. Naman, C.B., Puthiyedathu, S.T., Poulin, C.C., Poulin, R.X., 2023. Hidden in the photograph: The myth of complete metabolic coverage possible in metabolomics investigations. Anal. Sci. Adv. https://doi.org/10.1002/ansa.202200055
  6. Namitha, R., Abirami, B., Anoop, B.S., Dominic, D.V.D., Ameer, A., Manigundan, K., Radhakrishnan, M., Santhiya, P., Bhaskar, P. V., Govindaraju, K., Singh, I.S.B., Dharani, G., 2023. Synthesis and characterization of gold nanoparticles using Brevibacterium casei (SOSIST-06) isolated from Southern Ocean water samples and their in vitro and in silico anti-WSSV activity. Aquaculture 740205. https://doi.org/10.1016/j.aquaculture.2023.740205
  7. Raji Sathyan, K., Premraj, A., Thavarool Puthiyedathu, S., 2023. Characterisation of two tripartite motif ( <scp>TRIM</scp> ) containing genes from Asian seabass ( Lates calcarifer ) and their expression in response to virus infection and microbial molecular motifs. J. Aquat. Anim. Health. https://doi.org/10.1002/aah.10187
  1. Sathyan, N., Muhammed Musthafa, S., Anju, M.V., Archana, K., Athira, P.P., Namitha, P., Chaithanya, E.R., Priyaja, P., Bright Singh, I.S., Rosamma, P., 2022. Functional characterization of a histone H2A derived antimicrobial peptide HARRIOTTIN-1 from sicklefin chimaera, Neoharriotta pinnata. Dev. Comp. Immunol. 104554. https://doi.org/10.1016/j.dci.2022.104554 
  2. Athira, P.P., Anooja, V.V., Anju, M.V., Neelima, S., Archana, K., Muhammed Musthafa, S., Antony, S.P., Singh, I.S.B., Philip, R., 2022. A β-defensin isoform from the Flathead grey mullet, Mugil cephalus (Linnaeus 1758): Structural and functional characterisation in silico. Anim. Gene 25, 200128. https://doi.org/10.1016/j.angen.2022.200128
  3. Balakrishnan, S., Ameer, A., Pazhur Mohandas, S., Maliyekkal Sajeevan, A.K., Bhaskaran Sathyabhama, A., Singh, B., 2022. Pyocyanin as a safe aquaculture drug for the control of vibriosis in shrimp recirculating aquaculture system (RAS). Aquac. Int. 30, 2129–2144. https://doi.org/10.1007/s10499-022-00890-y
  4. Balakrishnan, S., Singh, I.S.B., Puthumana, J., 2022. Status in molluscan cell line development in last one decade (2010–2020): impediments and way forward. Cytotechnology, 74, 433–457. https://doi.org/10.1007/s10616-022-00539-x
  5. Geethakumari, D., Bhaskaran Sathyabhama, A., Raji Sathyan, K., Mohandas, D., Somasekharan, J. V., Thavarool Puthiyedathu, S., 2022. Folate functionalized chitosan nanoparticles as targeted delivery systems for improved anticancer efficiency of cytarabine in MCF-7 human breast cancer cell lines. Int. J. Biol. Macromol. 199, 150–161. https://doi.org/10.1016/j.ijbiomac.2021.12.070
  6. James, G., Das, B. C., Jose, S., & VJ, Rejish Kumar. 2021. Bacillus as an aquaculture friendly microbe. Aquaculture International, 1-31
  7. Krishna Priya, R. S., Premraj, A., Sajeevan, T.P., 2022. Antiviral radical SAM enzyme viperin homologue from Asian seabass (Lates calcarifer): Molecular characterisation and expression analysis. Dev. Comp. Immunol. 136, 104499. https://doi.org/10.1016/j.dci.2022.104499
  8. Krishna Priya, R.S., Premraj, A., Sivakumar, K.C., Sajeevan, T.P., 2022. Identification of two ISG15 homologues involved in host immune response against RGNNV in Asian seabass (Lates calcarifer). Fish Shellfish Immunol. Reports 3, 100054. https://doi.org/10.1016/j.fsirep.2022.100054
  9. Krishnapriya, R. S., Premraj, A., Sajeevan, T.P., 2022. Molecular characterization and expression analysis of two RING-between-RING (RBR) ubiquitin ligase orthologues from the Asian seabass (Lates calcarifer). Aquac. Int. 30, 1953-70 https://doi.org/10.1007/s10499-022-00883-x
  10. Krishnapriya, Raji Sathyan, Premraj, A., Thavarool Puthiyedathu, S., 2022. Molecular characterization and expression analysis of two type I interferons from Asian Seabass (Lates calcarifer) during nervous necrosis virus infection. Aquac. Res. [in press] https://doi.org/10.1111/are.15964
  11. Merlin, T.S., Umar, M., Dhaneesha, M., Joseph, V., Sajeevan, T.P., 2022. Screening and molecular identification of marine sponges with cytotoxic activities, collected from Gulf of Mannar, Indian Ocean. Indian J. Geo-Marine Sci. 51, 06, 529-535.
  12. Merlin, T.S., Umar, M., Puthiyedathu, S.T., 2022. Genomic insights into symbiosis and host adaptation of an ascidian-associated bacterium Bacillus aryabhattai MCCB 387. Symbiosis. [in press] https://doi.org/10.1007/s13199-022-00860-1
  13. Niveditha, L., Fu, P., Leao, T.F., Li, T., Wang, T., Poulin, R.X., Gaspar, L.R., Naman, C.B., Thavarool Puthiyedathu, S., 2022. Targeted Isolation of Two New Anti-inflammatory and UV-A Protective Dipyrroloquinones from the Sponge-associated Fungus Aspergillus tamarii MCCF102. Planta Med. 88, 774–782. https://doi.org/10.1055/a-1769-8480
  1. Ajitha, V., Sarasan, M., Parvathi Sreevidya, C., Aswathy, C., Kachiprath, B., Mohandas, A., Singh, I.S.B., Hameed, A.S.S., Schlenk, D., Magnuson, J.T., Puthumana, J., 2021a. Cytotoxic impacts of treated electroplating industrial effluent and the comparative effect of their metal components (Zn, Hg, and Zn+Hg) on Danio rerio gill (DrG) cell line. Sci. Total Environ. 793, 148533. https://doi.org/10.1016/j.scitotenv.2021.148533
  2. Ajitha, V., Sreevidya, C.P., Sarasan, M., Park, J.C., Mohandas, A., Singh, I.S.B., Puthumana, J., Lee, J.-S., 2021b. Effects of zinc and mercury on ROS-mediated oxidative stress-induced physiological impairments and antioxidant responses in the microalga Chlorella vulgaris. Environ. Sci. Pollut. Res. 28, 32475–32492. https://doi.org/10.1007/s11356-021-12950-6 
  3. Anoop, B.S., Puthumana, J., Vazhappilly, C.G., Kombiyil, S., Philip, R., Abdulaziz, A., Bright Singh, I.S., 2021. Immortalization of shrimp lymphoid cells by hybridizing with the continuous cell line Sf9 leading to the development of ‘PmLyO-Sf9 .’ Fish Shellfish Immunol. 113, 196–207. https://doi.org/10.1016/j.fsi.2021.03.023
  4. Archana, K., Sebastian, S.R., Sruthy, K.S., Nair, A., Anju, M.V., Bright Singh, I.S., Philip, R., 2021. Molecular and Functional Characterization of an Anti-lipopolysaccharide Factor Mm-ALF from Speckled Shrimp Metapenaeus monoceros. Probiotics Antimicrob. Proteins 13, 1183–1194. https://doi.org/10.1007/s12602-021-09741-3
  5. B.S., A., Puthumana, J., Sukumaran, V., Vazhappilly, C.G., Kombiyil, S., Philip, R., Singh, I.S.B., 2021. A Novel Approach of Transducing Recombinant Baculovirus into Primary Lymphoid Cells of Penaeus monodon for Developing Continuous Cell Line. Mar. Biotechnol. 23, 517–528. https://doi.org/10.1007/s10126-021-10043-6 
  6. Bhaskaran Sathyabhama, A., Puthumana, J., Kombiyil, S., Philip, R., Bright Singh, I.S., 2021. ‘PmLyO-Sf9 – WSSV complex’ could be a platform for elucidating the mechanism of viral entry, cellular apoptosis and replication impediments. Virology 553, 102–110. https://doi.org/10.1016/j.virol.2020.10.014
  7. Chandhini, S., Trumboo, B., Jose, S., Varghese, T., Rajesh, M., & Kumar, V. R. 2021 Insulin-like growth factor signalling and its significance as a biomarker in fish and shellfish research. Fish Physiology and Biochemistry, 1-21, 
  8. Dhaneesha, M., Umar, M., Merlin, T.S., Krishnan, K.P., Sukumaran, V., Sinha, R.K., Anas, A., Fu, P., MacMillan, J.B., Sajeevan, T.P., 2021. Pseudonocardia cytotoxica sp. nov., a novel actinomycete isolated from an Arctic fjord with potential to produce cytotoxic compound. Antonie Van Leeuwenhoek 114, 23–35. https://doi.org/10.1007/s10482-020-01490-7 
  9. Lakshmi, G., Beggi, F., Menta, C., Kumar, N.K., Jayesh, P., 2021. Dynamics of soil microarthropod populations affected by a combination of extreme climatic events in tropical home gardens of Kerala, India. Pedobiologia (Jena). 85–86, 150719. https://doi.org/10.1016/j.pedobi.2021.150719
  10. Joseph, V., Chellappan, G., Aparajitha, S., Ramya, R.N., Vrinda, S., Rejish Kumar, V.J., Bright Singh, I.S., 2021. Molecular characterization of bacteria and archaea in a bioaugmented zero-water exchange shrimp pond. SN Appl. Sci. 3, 458. https://doi.org/10.1007/s42452-021-04392-z
  11. Kachiprath, B., Nusaira, P., Raju, R., Puthumana, J., Jayanath, G., Mohan, A.S., Solomon, S., Antony, S.P., Philip, R., 2021. Screening of Arctic virome – A pilot study to delineate the viral diversity. J. Mar. Biol. Assoc. India 62, 99–105. https://doi.org/10.6024/jmbai.2020.62.2.2191-12 
  12. Kumaran, J., Poulose, S., Joseph, V., Bright Singh, I.S., 2021a. Enhanced biomass production and proximate composition of marine microalga Nannochloropsis oceanica by optimization of medium composition and culture conditions using response surface methodology. Anim. Feed Sci. Technol. 271, 114761. https://doi.org/10.1016/j.anifeedsci.2020.114761
  13. Kumaran, J., Singh, I.S.B., Joseph, V., 2021b. Effective biomass harvesting of marine diatom Chaetoceros muelleri by chitosan-induced flocculation, preservation of biomass, and recycling of culture medium for aquaculture feed application. J. Appl. Phycol. 33, 1605–1619. https://doi.org/10.1007/s10811-021-02369-4
  14. Lakshmi, G., Beggi, F., Menta, C., Kumar, N.K., Jayesh, P., 2021. Dynamics of soil microarthropod populations affected by a combination of extreme climatic events in tropical home gardens of Kerala, India. Pedobiologia (Jena). 85–86, 150719. https://doi.org/10.1016/j.pedobi.2021.150719
  15. Mohandas, S.P., Balan, L., Gopi, J., Anoop, B.S.S., Mohan P., S., Philip, R., Cubelio, S.S., Singh, I.S.B.S.B., Mohan P, S., Philip, R., Cubelio, S.S., Singh, I.S.B.S.B., 2021. Biocompatibility of polyhydroxybutyrate-co-hydroxyvalerate films generated from Bacillus cereus MCCB 281 for medical applications. Int. J. Biol. Macromol. 176, 244–252. https://doi.org/10.1016/j.ijbiomac.2021.02.006 
  16. Neelima, S., Archana, K., Athira, P.P., Anju, M. V., Anooja, V. V., Bright Singh, I.S., Philip, R., 2021. Molecular characterization of a novel β-defensin isoform from the red-toothed trigger fish, Odonus niger (Ruppel, 1836). J. Genet. Eng. Biotechnol. 19, 71. https://doi.org/10.1186/s43141-021-00175-6
  17. Preena, P.G., Rejish Kumar, V.J., Singh, I.S.B., 2021. Nitrification and denitrification in recirculating aquaculture systems: the processes and players. Rev. Aquac. 13, 2053–2075. https://doi.org/10.1111/raq.12558
  18. Sarasan, M., Job, N., Puthumana, J., Ravinesh, R., Kachiprath, B., Philip, R., 2021. Enzyme profiling of macroalgal endophytes: An attempt to uncover the arsenal of novel biocatalysts. J. Mar. Biol. Assoc. India 62, 112–116. https://doi.org/10.6024/jmbai.2020.62.2.2183-14 
  19. Sivakumar, K.C., Thavarool Puthiyedathu, S., 2021. Challenges and prospects of Viral Envelope protein VP28‐based control strategies to combat white spot syndrome virus in penaeid shrimps: a review. Rev. Aquac. 13, 734–743. https://doi.org/10.1111/raq.12497
  20. Sunish, K.S., Sreedharan, P., Daniel, S., Biji, M., Rosamma, P., Sukumaran, V., Mohandas, A., Singh, I.S.B., 2021. A novel substituted derivative of sterol from marine actinomycetes Nocardiopsis alba MCCB 110 antagonistic to the aquaculture pathogen Vibrio harveyi. Microb. Pathog. 157, 104967. https://doi.org/10.1016/j.micpath.2021.104967
  21. Umar, M., Krishnan, K.P., Sinha, R.K., Kannukkarathi, T., Merlin, T.S., Johnson, J.I., Joseph, V., Puthiyedathu, S.T., 2021. Roseitranquillus sediminis gen. nov., sp. nov. a novel genus and species of the family Rhodobacteraceae, isolated from sediment of an Arctic fjord. Antonie Van Leeuwenhoek 114, 2147–2162. https://doi.org/10.1007/s10482-021-01669-6
  22. Vijayan, A., Vattiringal Jayadradhan, R.K., Pillai, D., Prasannan Geetha, P., Joseph, V., Isaac Sarojini, B.S., 2021. Nitrospira as versatile nitrifiers: Taxonomy, ecophysiology, genome characteristics, growth, and metabolic diversity. J. Basic Microbiol. 61, 88–109. https://doi.org/10.1002/jobm.202000485 
  1. Leber CA, C. Benjamin Naman , Lena Keller, JehadAlmaliti, Eduardo J. E. Caro-Diaz,, EvgeniaGlukhov,, Valsamma Joseph, Sajeevan TP, Andres Joshua Reyes, Jason S. Biggs, Te Li, Ye Yuan, Shan He, Xiaojun Yan. and William H. Gerwick . 2020. Applying a Chemogeographic Strategy for Natural Product Discovery from the Marine Cyanobacterium Moorena bouillonii. Marine Drugs, 18, 515; doi:10.3390/md18100515
  2. Jose, D., Preena, P.G., Kumar, V.J.R., Philip, R., Singh, I.S.B., 2020. Metaproteomic insights into ammonia oxidising bacterial consortium developed for bioaugmenting nitrification in aquaculture systems. Biologia (Bratisl). 75, 1751–1757. https://doi.org/10.2478/s11756-020-00481-3
  3. Lekshmi, N , Umar, MD, Dhaneesha, M, Rojin Joseph, Ravinesh, R. And Sajeevan, T.P. 2020 Endophytic Fungi Isolated from the Marine Sponges as a Source of Potential Bioactive Compounds Journal of Aquatic Biology & Fisheries (8) pp. 63-71
  4. Nair, R.R., Rangaswamy, B., Sarojini, B.S.I., Joseph, V., Boobal, R., Bright Singh, I.S., Joseph, V., 2020. Anaerobic ammonia-oxidizing bacteria in tropical bioaugmented zero water exchange aquaculture ponds. Environ. Sci. Pollut. Res. 27, 10541–10552. https://doi.org/10.1007/s11356-020-07663-1
  5. Preena, P.G., Swaminathan, T.R., Kumar, V.J.R., Singh, I.S.B., 2020. Antimicrobial resistance in aquaculture: a crisis for concern. Biologia (Bratisl). 75, 1497–1517. https://doi.org/10.2478/s11756-020-00456-4
  6. Preena, P.G., Swaminathan, T.R., Rejish Kumar, V.J., Bright Singh, I.S., 2020. Unravelling the menace: detection of antimicrobial resistance in aquaculture. Lett. Appl. Microbiol. 71, 26–38. https://doi.org/10.1111/lam.13292
  7. Sarasan, M., Job, N., Puthumana, J., R, R., M P, P., Thomas, L.C., Philip, R., 2020. Exploration and profiling of hidden endophytic mycota of marine macroalgae with potential drug leads. FEMS Microbiol. Lett. 367, fnaa078. https://doi.org/10.1093/femsle/fnaa078
  8. Sivakumar, K.C., Haixiao, J., Naman, C.B., Sajeevan, T.P., 2020. Prospects of multitarget drug designing strategies by linking molecular docking and molecular dynamics to explore the protein–ligand recognition process. Drug Dev. Res. 81, 685–699. https://doi.org/10.1002/ddr.21673 
  9. Sunish, K.S., Biji, M., Rosamma, P., Sudheer, N.S., Sreedharan, K., Mohandas, A., Singh, I.S.B., 2020. Marine actinomycetes Nocardiopsis alba MCCB 110 has immunomodulatory property in the tiger shrimp Penaeus monodon. Fish Shellfish Immunol. 102, 125–132. https://doi.org/10.1016/j.fsi.2020.03.069
  1. Ajitha, V., Sreevidya, C.P., Kim, J.H., Bright Singh, I.S., Mohandas, A., Lee, J.-S., Puthumana, J., 2019. Effect of metals of treated electroplating industrial effluents on antioxidant defense system in the microalga Chlorella vulgaris. Aquat. Toxicol. 217, 105317. https://doi.org/10.1016/j.aquatox.2019.105317
  2. Anju, A., Smitha, C.K., Preetha, K., Boobal, R., Rosamma, P., 2019. Molecular characterization, recombinant expression and bioactivity profile of an antimicrobial peptide, Ss-arasin from the Indian mud crab, Scylla serrata. Fish Shellfish Immunol. 88, 352–358. https://doi.org/10.1016/j.fsi.2019.03.007
  3. Li, Wang, Zhang, Zhang, Sajeevan, Joseph, Armstrong, He, Yan, Naman, 2019. A Systematic Review of Recently Reported Marine Derived Natural Product Kinase Inhibitors. Mar. Drugs 17, 493. https://doi.org/10.3390/md17090493 
  4. Preena, P.G., Achuthan, C., Kumar, V.J.R., Boobal, R., Deepa, G.D., Puthumana, J., Poulose, S., Surekhamol, I.S., Singh, I.S.B., 2019. Community composition of marine and brackish water ammonia-oxidizing consortia developed for aquaculture application. Water Sci. Technol. 79, 1017–1028. https://doi.org/10.2166/wst.2019.029 
  5. Sabu, S., Singh, I.S.B., Joseph, V., 2019. Improved lipid production in oleaginous brackish diatom Navicula phyllepta MACC8 using two-stage cultivation approach. 3 Biotech 9, 437. https://doi.org/10.1007/s13205-019-1968-1
  6. Sruthy, K.S., Nair, A., Antony, S.P., Puthumana, J., Singh, I.S.B., Philip, R., 2019a. A histone H2A derived antimicrobial peptide, Fi-Histin from the Indian White shrimp, Fenneropenaeus indicus: Molecular and functional characterization. Fish Shellfish Immunol. 92, 667–679. https://doi.org/10.1016/j.fsi.2019.06.044 
  7. Sruthy, K.S., Nair, A., Antony, S.P., Puthumana, J., Singh, I.S.B., Philip, R., P, J., Singh, I.S.B., Philip, R., Puthumana, J., Singh, I.S.B., Philip, R., 2019b. A histone H2A derived antimicrobial peptide, Fi-Histin from the Indian White shrimp, Fenneropenaeus indicus: Molecular and functional characterization. Fish Shellfish Immunol. 92, 667–679. https://doi.org/10.1016/j.fsi.2019.06.044
  8. Vrinda, S., Abdulaziz, A., Abhilash, K.S., Jasmin, C., Kripa, V., Bright Singh, I.S., 2019. Neuroendocrine and immunotoxicity of polyaromatic hydrocarbon, chrysene in crustacean post larvae. Ecotoxicology. https://doi.org/10.1007/s10646-019-02094-2
  1. Babu, D.T., Archana, K., Kachiprath, B., Solomon, S., Jayanath, G., Singh, I.S.B., Philip, R., 2018. Marine actinomycetes as bioremediators in Penaeus monodon rearing system. Fish Shellfish Immunol. 75, 231–242. https://doi.org/10.1016/j.fsi.2018.01.037
  2. Deepa, G., Sivakumar, K.C., Sajeevan, T.P., 2018. Molecular simulation and in vitro evaluation of chitosan nanoparticles as drug delivery systems for the controlled release of anticancer drug cytarabine against solid tumours. 3 Biotech 8, 493. https://doi.org/10.1007/s13205-018-1510-x 
  3. Dhaneesha, M., Hasin, O., Sivakumar, K.C., Ravinesh, R., Naman, C.B., Carmeli, S., Sajeevan, T.P., 2018. DNA Binding and Molecular Dynamic Studies of Polycyclic Tetramate Macrolactams (PTM) with Potential Anticancer Activity Isolated from a Sponge-Associated Streptomyces zhaozhouensis subsp. mycale subsp. nov. Mar. Biotechnol. https://doi.org/10.1007/s10126-018-9866-9 
  4. Jayanath, G., Mohandas, S.P., Kachiprath, B., Solomon, S., Sajeevan, T.P., Bright Singh, I.S., Philip, R., 2018. A novel solvent tolerant esterase of GDSGG motif subfamily from solar saltern through metagenomic approach: Recombinant expression and characterization. Int. J. Biol. Macromol. 119, 393–401. https://doi.org/10.1016/j.ijbiomac.2018.06.057 
  5. Jose, D., Mohandas, A., Bright Singh, I.S., 2018a. Bioprocess technology for LasB protease (elastase) production from Pseudomonas aeruginosa MCCB 123. Indian J. Biotechnol. 17, 284–289
  6. Jose, D., Mohandas, A., Bright Singh, I.S., 2018b. A Non-Pathogenic Environmental Isolate of Pseudomonas aeruginosa MCCB 123 with Biotechnological Potential. Int. J. Curr. Microbiol. Appl. Sci. 7, 3060–3071. https://doi.org/10.20546/ijcmas.2018.701.363
  7. Kachiprath, B., Puthumana, J., Gopi, J., Solomon, S., Krishnan, K.P., Philip, R., 2018. Amplicon sequencing based profiling of bacterial diversity from Krossfjorden, Arctic. Data Br. https://doi.org/10.1016/j.dib.2018.11.101  
  8. Mohan, A.S., Nusaira, P., Raju, R., Puthumana, J., Kachiprath, B., Solomon, S., Jayanath, G., Philip, R., 2018. Bacterial Communities from near Glacier and Transition Zones of Kongsfjorden, Arctic: A Metagenomic Study. Indian J. Sci. Res. 20, 56–62 
  9. Mohandas, S.P., Balan, L., Jayanath, G., Anoop, B.S.S., Philip, R., Cubelio, S.S., Bright Singh, I.S., 2018. Biosynthesis and characterization of polyhydroxyalkanoate from marine Bacillus cereus MCCB 281 utilizing glycerol as carbon source. Int. J. Biol. Macromol. 119, 380–392. https://doi.org/10.1016/j.ijbiomac.2018.07.044 
  10. Nair, R.R., Boobal, R., Vrinda, S., Bright Singh, I.S., Valsamma, J., 2018. Ammonia-oxidizing bacterial and archaeal communities in tropical bioaugmented zero water exchange shrimp production systems. J. Soils Sediments. https://doi.org/10.1007/s11368-018-2185-y 
  11. Preena, P.G., Kumar, V.J.R., Achuthan, C., George, R., Boobal, R., Nair, R.R., Singh, I.S.B., 2018. Diversity of marine and brackish water nitrite-oxidizing consortia developed for activating nitrifying bioreactors in aquaculture. Int. J. Environ. Sci. Technol. 15, 2399–2410. https://doi.org/10.1007/s13762-017-1580-z
  12. Saheeda, P., Sabira, K., Dhaneesha, M., Jayaleksmi, S., 2018. Investigation on the pH-independent photoluminescence emission from carbon dots impregnated on polymer matrix. Luminescence 33, 22–28. https://doi.org/10.1002/bio.3367 
  13. Vrinda, S., Jasmin, C., Philip, R., Bright Singh, I.S., 2018. Thioredoxin fused CHH1 protein as antigen for polyclonal antisera: Application to regulate glycemia in Penaeus monodon. Indian J. Exp. Biol. 56, 83–92. 
  1. Dhaneesha, M., Benjamin Naman, C., Krishnan, K.P., Sinha, R.K., Jayesh, P., Joseph, V., Bright Singh, I.S., Gerwick, W.H., Sajeevan, T.P., 2017. Streptomyces artemisiae MCCB 248 isolated from Arctic fjord sediments has unique PKS and NRPS biosynthetic genes and produces potential new anticancer natural products. 3 Biotech 7, 32. https://doi.org/10.1007/s13205-017-0610-3
  2. Jose, D., Jayesh, P., Gopinath, P., Mohandas, A., Bright Singh, I.S., 2017a. A rapid two step bacterial DNA extraction method using LasA protease of Pseudomonas aeruginosa MCCB 123. Indian J. Biotechnol. 16, 495–504. https://doi.org/10.1007/s13205-017-0610-3
  3. Jose, D., Manjusha, K., Jose, S., Mohandas, A., Bright Singh, I.S., 2017b. Purification and characterization of highly active LasB protease from Pseudomonas aeruginosa MCCB 123. Indian J. Exp. Biol. 55, 303–310.
  4. Joy, S., Alikunju, A.P., Jose, J., Sudha, H.S.H., Parambath, P.M., Puthiyedathu, S.T., Philip, B., 2017. Oxidative stress and antioxidant defense responses of Etroplus suratensis to acute temperature fluctuations. J. Therm. Biol. 70, 20–26. https://doi.org/10.1016/j.jtherbio.2017.10.010 
  5. Kumaran, J., Jose, B., Joseph, V., Bright Singh, I.S., 2017. Optimization of growth requirements of marine diatom Chaetoceros muelleri using Response Surface Methodology. Aquac. Res. 48, 1513–1524. https://doi.org/10.1111/are.12987 
  6. Mohandas, S.P., Balan, L., Lekshmi, N., Cubelio, S.S., Philip, R., Bright Singh, I.S., 2017. Production and characterization of polyhydroxybutyrate from Vibrio harveyi MCCB 284 utilizing glycerol as carbon source. J. Appl. Microbiol. 122, 698–707. https://doi.org/10.1111/jam.13359 
  7. Preena, P.G., Manju, N.J., Deepesh, V., Thomas, A., Bright Singh, I.S., 2017. Genetic diversity of nitrate reducing bacteria in marine and brackish water nitrifying bacterial consortia generated for activating nitrifying bioreactors in recirculating aquaculture systems. Aquac. Res. 48, 5729–5740. https://doi.org/10.1111/are.13396 
  8. Sabu, S., Bright Singh, I.S., Joseph, V., 2017a. Molecular Identification and Comparative Evaluation of Tropical Marine Microalgae for Biodiesel Production. Mar. Biotechnol. 19, 328–344. https://doi.org/10.1007/s10126-017-9754-8 
  9. Sabu, S., Singh, I.S.B., Joseph, V., 2017b. Optimisation of critical medium components and culture conditions for enhanced biomass and lipid production in the oleaginous diatom Navicula phyllepta: a statistical approach. Environ. Sci. Pollut. Res. 24, 26763–26777. https://doi.org/10.1007/s11356-017-0274-x
  10. Sukumaran, V., Jose, S., Chekidhenkuzhiyil, J., Lalithabai, M.D., Nair, S.B., Christo, D., Kizhakedath, V.K., Paran, B.C., Philip, R., Sarojini, B.S.I., 2017. ELISA detection of vitellogenin in the haemolymph of Penaeus monodon (Fabricius, 1798) (Caridea: Penaeidae): indirect indicator of female maturity. J. Crustac. Biol. 37, 473–480. https://doi.org/10.1093/jcbiol/rux040 
  11. Vrinda S, Reshmi C, Jose S, Reynold P, Vijayan KK, Philip R, Singh IS. 2017 Crustacean hyperglycemic hormone family gene silencing in Penaeus monodon mediated through dsRNA synthesized in vitro from genomic and cDNA. Indian Journal of Biotechnology  16(1): 37-43, 
  12. Vrinda, S., Jasmin, C., Sivakumar, K.C., Jose, S., Jose, B., Philip, R., Bright Singh, I.S., 2017. Regulating gonad inhibition and vitellogenin/vitellin induction in Penaeus monodon using mature GIH fusion protein and polyclonal antisera. Comp. Biochem. Physiol. Part A Mol. Integr. Physiol. 203, 167–178. https://doi.org/10.1016/j.cbpa.2016.09.007 
  1. Afsal, V. V., Antony, S.P., Philip, R., Bright Singh, I.S., 2016. Molecular Characterization of a Newly Identified Subfamily Member of Penaeidin from two Penaeid Shrimps, Fenneropenaeus indicus and Metapenaeus monoceros. Probiotics Antimicrob. Proteins 8, 46–52. https://doi.org/10.1007/s12602-015-9203-9
  2. Arshad, E., Anas, A., Asok, A., Jasmin, C., Pai, S.S., Bright Singh, I.S., Mohandas, A., Biju, V., 2016. Fluorescence detection of the pathogenic bacteria Vibrio harveyi in solution and animal cells using semiconductor quantum dots. RSC Adv. 6, 15686–15693. https://doi.org/10.1039/C5RA24161H
  3. Asha, P., Divya, J., Bright Singh, I.S., 2016. Purification and characterisation of processive-type endoglucanase and β-glucosidase from Aspergillus ochraceus MTCC 1810 through saccharification of delignified coir pith to glucose. Bioresour. Technol. 213, 245–248. https://doi.org/10.1016/j.biortech.2016.03.013 
  4. Bright Singh, I.S., George, R., Jose, B., Sugunan, S., 2016. Preventive healthcare in Penaeus monodon under zero water exchange mode supported with bioaugmentors, probiotics and biological response modifiers. Fish Shellfish Immunol. 53, 61. https://doi.org/10.1016/j.fsi.2016.03.050 
  5. Jayesh, P., Vrinda, S., Priyaja, P., Philip, R., Singh, I.S.B., 2016. Impaired telomerase activity hinders proliferation and in vitro transformation of Penaeus monodon lymphoid cells. Cytotechnology 68, 1301–1314. https://doi.org/10.1007/s10616-015-9890-9 
  6. Neelanchal, V., Sajeevan, T.P., 2016. Screening marine bacterium strains of Actinomycetes as antagonists to pathogenic Vibrios, molecular identification of potent strain and media optimization by: Plackett-Burman analysis and response surface methodology. Invertis J. Sci. Technol. 9, 79–93. https://doi.org/10.5958/2454-762X.2016.00003.2  
  7. Priyaja, P., Jayesh, P., Haseeb, M., Jose, B., Philip, R., Singh, I.S.B., 2016a. Evaluation of pyocyanin toxicity in various life stages of Penaeus monodon and in nitrifying bacterial consortia for their safe application in recirculating aquaculture systems (RAS) to abrogate pathogenic vibrios. Aquac. Int. https://doi.org/10.1007/s10499-016-0075-0 
  8. Priyaja, P., Jayesh, P., Philip, R., Bright Singh, I.S., 2016b. Pyocyanin induced in vitro oxidative damage and its toxicity level in human, fish and insect cell lines for its selective biological applications. Cytotechnology 68, 143–155. https://doi.org/10.1007/s10616-014-9765-5  view
  9. Puthumana, J., Philip, R., Bright Singh, I.S., 2016. Transgene expression in Penaeus monodon cells: evaluation of recombinant baculoviral vectors with shrimp specific hybrid promoters. Cytotechnology 68, 1147–1159. https://doi.org/10.1007/s10616-015-9872-y
  10. Sivakumar, K.C., Sajeevan, T.P., Bright Singh, I.S., 2016. Marine derived compounds as binders of the White spot syndrome virus VP28 envelope protein: In silico insights from molecular dynamics and binding free energy calculations. Comput. Biol. Chem. 64, 359–367. https://doi.org/10.1016/j.compbiolchem.2016.08.006 
  11. Solomon, S., Kachiprath, B., Jayanath, G., Sajeevan, T.P., Bright Singh, I.S., Philip, R., 2016. High-quality metagenomic DNA from marine sediment samples for genomic studies through a preprocessing approach. 3 Biotech 6, 160. https://doi.org/10.1007/s13205-016-0482-y 
  12. Thomas, A., Sudheer, N.S., Kiron, V., Bright Singh, I.S., Narayanan, R.B., 2016. Expression profile of key immune-related genes in Penaeus monodon juveniles after oral administration of recombinant envelope protein VP28 of white spot syndrome virus. Microb. Pathog. 96, 72–79. https://doi.org/10.1016/j.micpath.2016.05.002 
  13. Vrinda, S., Jasmin, C., Sivakumar, K.C., Jose, B., Philip, R., Bright Singh, I.S., 2016. Moult-inhibiting fusion protein augments while polyclonal antisera attenuate moult stages and duration in Penaeus monodon. Gen. Comp. Endocrinol. 233, 32–42. https://doi.org/10.1016/j.ygcen.2016.05.012

 

  1. Jayesh, P., Philip, R., Singh, I.S.B., 2015. Multifactorial interaction of growth factors on Penaeus monodon lymphoid cells and the impact of IGFs in DNA synthesis and metabolic activity in vitro. Cytotechnology 67, 559–571. https://doi.org/10.1007/s10616-014-9697-0
  2. Preetha, R., Vijayan, K.K., Jayaprakash, N.S., Alavandi, S. V., Santiago, T.C., Bright Singh, I.S., 2015. Optimization of Culture Conditions for Mass Production of the Probiotics Pseudomonas MCCB 102 and 103 Antagonistic to Pathogenic Vibrios in Aquaculture. Probiotics Antimicrob. Proteins 7, 137–145. https://doi.org/10.1007/s12602-015-9185-7
  3. Puthumana J, Jose S, Philip R, Singh IB. 2015 Cellular and molecular markers in monitoring the fate of lymphoid cell culture from Penaeus monodon Fabricius (1798). Fish & shellfish immunology. 1;47(2):893-901, 
  4. Puthumana, J., Prabhakaran, P., Philip, R., Singh, I.S.B., 2015. Attempts on producing lymphoid cell line from Penaeus monodon by induction with SV40-T and 12S EIA oncogenes. Fish Shellfish Immunol. 47, 655–663. https://doi.org/10.1016/j.fsi.2015.08.010 
  5. Sruthy, K.S., Chaithanya, E.R., Sathyan, N., Nair, A., Antony, S.P., Bright Singh, I.S., Philip, R., 2015a. Molecular Characterization and Phylogenetic Analysis of Novel Isoform of Anti-lipopolysaccharide Factor from the Mantis Shrimp, Miyakea nepa. Probiotics Antimicrob. Proteins 7, 275–283. https://doi.org/10.1007/s12602-015-9198-2
  6. Sruthy, K.S., Nair, A., Cubelio, S.S., Bright Singh, I.S., Philip, R., 2015b. Molecular characterization and phylogenetic analysis of an anti-lipopolysaccharide factor from the crucifix crab, Charybdis feriatus. Open Access Anim. Physiol. 149. https://doi.org/10.2147/OAAP.S84508 
  7. Sudheer, N.S., Poulose, G., Thomas, A., Viswanath, K., Kulkarni, A., Narayanan, R.B., Philip, R., Singh, I.S.B., 2015. Expression profile of bio-defense genes in Penaeus monodon gills in response to formalin inactivated white spot syndrome virus vaccine. Antiviral Res. 117, 60–68. https://doi.org/10.1016/j.antiviral.2015.02.008 
  8. Wilson, W., Lowman, D.W., Antony, S.P., Jayesh, P., Bright Singh, I.S., Philip, R., 2015. Immune gene expression profile of Penaeus monodon in response to marine yeast glucan application and white spot syndrome virus challenge. Fish Shellfish Immunol. 43, 346–356. https://doi.org/10.1016/j.fsi.2014.12.032 
  1. Jose, D., Jayesh, P., Gopinath, P., Mohandas, A., Bright Singh, I.S., 2014. Potential application of beta-1, 3 glucanase from an environmental isolate of Pseudomonas aeruginosa MCCB 123 in fungal DNA extraction. Indian J. Exp. Biol. 52, 89–96. 
  2. Joseph, V., Haseeb, M., Ranjit, S., Anas, A., Bright Singh, I.S., 2014. Shrimp Production under Zero Water Exchange Mode Coupled with Bioremediation and Application of Probiotics. J. Fish. Int. 9, 5–14.
  3. Karthik, V., Kamalakannan, V., Thomas, A., Sudheer, N.S., Bright Singh, I.S., Narayanan, R.B., 2014. Functional Characterization of a c-type Lysozyme from Indian Shrimp Fenneropenaeus indicus. Probiotics Antimicrob. Proteins 6, 114–121. https://doi.org/10.1007/s12602-013-9146-y 
  4. Kulkarni, A.D., Kiron, V., Rombout, J.H.W.M., Brinchmann, M.F., Fernandes, J.M.O., Sudheer, N.S., Bright Singh, I.S., 2014. Protein profiling in the gut of Penaeus monodon gavaged with oral WSSV-vaccines and live white spot syndrome virus. Proteomics 14, 1660–1673. https://doi.org/10.1002/pmic.201300405 
  5. Poulose, S., Bright Singh, I.S., 2014. Optimization of culture conditions for the production of single cell protein from marine yeast Candida MCCF 101 as feed supplement in aquaculture. J. Aquat. Biol. Fish. 2, 283–289. 
  6. Rejish Kumar, V.J., Joseph, V., Philip, R., Singh, I.S.B., 2014. Nitrification by Stringed Bed Suspended Bioreactors at Different Salinities and Substrate Concentrations. Indian J. Geo-Marine Sci. 43. 
  7. Surekhamol, I.S., Deepa, G.D., Somnath Pai, S., Sreelakshmi, B., Varghese, S., Bright Singh, I.S., 2014. Isolation and characterization of broad spectrum bacteriophages lytic to Vibrio harveyi from shrimp farms of Kerala, India. Lett. Appl. Microbiol. 58, 197–204. https://doi.org/10.1111/lam.12175 
  8. Syamkumar, R., Rojith, G., Rajathy, S., Bright Singh, I.S., 2014. Phytotoxicity assessment of coir pith effluent generated during lignin recovery process. Res. J. Chem. Sci. 4, 17–21. 
  9. Thomas, A., Sudheer, N.S., Viswanathan, K., Kiron, V., Bright Singh, I.S., Narayanan, R.B., 2014. Immunogenicity and protective efficacy of a major White Spot Syndrome Virus (WSSV) envelope protein VP24 expressed in Escherichia coli against WSSV. J. Invertebr. Pathol. 123, 17–24. https://doi.org/10.1016/j.jip.2014.08.004 
  1. Chaithanya, E.R., Philip, R., Sathyan, N., Anil Kumar, P.R., Antony, S.P., Sanjeevan, V.N., Bright Singh, I.S., 2013a. A Novel Isoform of the Hepatic Antimicrobial Peptide, Hepcidin (Hepc-CB1), from a Deep-Sea Fish, the Spinyjaw Greeneye Chlorophthalmus bicornis (Norman, 1939): Molecular Characterisation and Phylogeny. Probiotics Antimicrob. Proteins 5, 1–7. https://doi.org/10.1007/s12602-012-9120-0 
  2. Chaithanya, E.R., Philip, R., Sathyan, N., Anil Kumar, P.R., Cubelio, S.S., Bright Singh, I.S., 2013b. Molecular Characterisation and Phylogenetic Analysis of a Novel Isoform of Hepatic Antimicrobial Peptide, Hepcidin (Zc-hepc1), from the Coral Fish Moorish idol, Zanclus cornutus (Linnaeus, 1758). Probiotics Antimicrob. Proteins 5, 187–194. https://doi.org/10.1007/s12602-013-9139-x 
  3. Jacob, J.C., Ramya, K.D., Bright Singh, I.S., Philip, R., 2013. Bathymetric and latitudinal shift in sedimentary parameters along the shelf sediments of Bay of Bengal: Influence on heterotrophic bacteria. Adv. Appl. Sci. Res. 4, 119–133. 
  4. Jayesh, P., Jose, S., Philip, R., Bright Singh, I.S., 2013. A novel medium for the development of in vitro cell culture system from Penaeus monodon. Cytotechnology 65, 307–322. https://doi.org/10.1007/s10616-012-9491-9
  5. Kulkarni, A.D., Rombout, J.H.W.M., Bright Singh, I.S., Sudheer, N.S., Vlak, J.M.M., Caipang, C.M.A., Brinchmann, M.F., Kiron, V., 2013. Truncated VP28 as oral vaccine candidate against WSSV infection in shrimp: An uptake and processing study in the midgut of Penaeus monodon. Fish Shellfish Immunol. 34, 159–166. https://doi.org/10.1016/j.fsi.2012.10.028 
  6. Kutty, S.N., Lawman, D., Bright Singh, I.S., Philip, R., 2013. Black yeasts from the slope sediments of Bay of Bengal : phylogenetic and functional characterization. Mycosphere 4, 346–361.
  7. Manjusha, K., Jayesh, P., Jose, D., Sreelakshmi, B., Priyaja, P., Gopinath, P., Saramma, A. V., Bright Singh, I.S., 2013. Alkaline protease from a non-toxigenic mangrove isolate of Vibrio sp. V26 with potential application in animal cell culture. Cytotechnology 65, 199–212. https://doi.org/10.1007/s10616-012-9472-z 
  8. Ramya, K.D., Jacob, J.C., Correya, N.S., Bright Singh, I.S., Philip, R.,  2013. Biogeochemistry of the shelf sediments of south eastern Arabian sea: Effect on benthic bacterial heterotrophs. Adv. Appl. Sci. Res. 4, 315–328. 
  9. Rejish Kumar, V.J., Sukumaran, V., Achuthan, C., Joseph, V., Philip, R., Bright Singh, I.S., 2013. Molecular characterization of the nitrifying bacterial consortia employed for the activation of bioreactors used in brackish and marine aquaculture systems. Int. Biodeterior. Biodegradation 78, 74–81. https://doi.org/10.1016/j.ibiod.2013.01.002 
  10. Rojith, G., Bright Singh, I.S., 2013a. Hydrogen peroxide pretreatment efficiency comparison and characterization of lignin recovered from coir pith black liquor. J. Environ. Res. Dev. 7, 1333–1339. 
  11. Rojith, G., Bright Singh, I.S., 2013b. Cellulose crystallanity change assessment of Biochar produced by pyrolysis of Coir pith. Res. J. Recent Sci. 2, 1–6. 
  12. Sathyan, N., Philip, R., Chaithanya, E.R., Anil Kumar, P.R., Sanjeevan, V.N., Singh, I.S.B., 2013. Characterization of Histone H2A Derived Antimicrobial Peptides, Harriottins, from Sicklefin Chimaera Neoharriotta pinnata (Schnakenbeck, 1931) and Its Evolutionary Divergence with respect to CO1 and Histone H2A. ISRN Mol. Biol. 2013, 1–10. https://doi.org/10.1155/2013/930216  
  13. Sreedharan, K., Philip, R., Singh, I.S.B., 2013. Characterization and virulence potential of phenotypically diverse Aeromonas veronii isolates recovered from moribund freshwater ornamental fishes of Kerala, India. Antonie Van Leeuwenhoek 103, 53–67. https://doi.org/10.1007/s10482-012-9786-z 
  1. Afsal, V. V., Antony, S.P., Chaithanya, E.R., Bright Singh, I.S., Philip, R., 2012a. Two isoforms of anti-lipopolysaccharide factors identified and characterized from the hemocytes of portunid crabs, Portunus pelagicus and Scylla tranquebarica. Mol. Immunol. 52, 258–263. https://doi.org/10.1016/j.molimm.2012.06.003 
  2. Afsal, V. V., Antony, S.P., Sanjeevan, V.N., Anil Kumar, P.R., Bright Singh, I.S., Philip, R., 2012b. A new isoform of anti-lipopolysaccharide factor identified from the blue swimmer crab, Portunus pelagicus: Molecular characteristics and phylogeny. Aquaculture 356–357, 119–122. https://doi.org/10.1016/j.aquaculture.2012.05.028 
  3. Asok, A., Arshad, E., Jasmin, C., Somnath Pai, S., Bright Singh, I.S., Mohandas, A., Anas, A., 2012. Reducing Vibrio load in Artemia nauplii using antimicrobial photodynamic therapy: a promising strategy to reduce antibiotic application in shrimp larviculture. Microb. Biotechnol. 5, 59–68. https://doi.org/10.1111/j.1751-7915.2011.00297.x 
  4. Jayesh, P., Seena, J., Bright Singh, I.S., 2012. Establishment of Shrimp Cell Lines: Perception and Orientation. Indian J. Virol. 23, 244–251. https://doi.org/10.1007/s13337-012-0089-9 
  5. Jose, S., Jayesh, P., Sudheer, N.S., Poulose, G., Mohandas, A., Philip, R., Bright Singh, I.S., 2012. Lymphoid organ cell culture system from Penaeus monodon (Fabricius) as a platform for white spot syndrome virus and shrimp immune-related gene expression. J. Fish Dis. 35, 321–334. https://doi.org/10.1111/j.1365-2761.2012.01348.x 
  6. Sathyan, N., Philip, R., Chaithanya, E.R., Anil Kumar, P.R., 2012. Identification and Molecular Characterization of Molluskin, a Histone-H2A-Derived Antimicrobial Peptide from Molluscs. ISRN Mol. Biol. 2012, 1–6. https://doi.org/10.5402/2012/219656 
  7. Smitha, S., Haseena, V.S., Narayanan, T.N., Reena Mary, A.P., Ajayan, P.M., Puthumana, J., Aziz, A.A., Aburto, R.R., Mani, S.A., Joy, P.A., Anantharaman, M.R., 2012. Fluorescent Superparamagnetic Iron Oxide Core–Shell Nanoprobes for Multimodal Cellular Imaging. Mater. Express 2, 265–274. https://doi.org/10.1166/mex.2012.1083 
  8. Sreedharan, K., Philip, R., Singh, I.S.B., 2012. Virulence potential and antibiotic susceptibility pattern of motile aeromonads associated with freshwater ornamental fish culture systems: a possible threat to public health. Brazilian J. Microbiol. 43, 754–765. https://doi.org/10.1590/S1517-83822012000200040 
  9. Sudheer, N.S., Philip, R., Bright Singh, I.S., 2012. Anti-white spot syndrome virus activity of Ceriops tagal aqueous extract in giant tiger shrimp Penaeus monodon. Arch. Virol. 157, 1665–1675. https://doi.org/10.1007/s00705-012-1346-3 
  1. Antony, S.P., Bright Singh, I.S., Jose, R.M., Anil Kumar, P.R., Philip, R., 2011a. Antimicrobial peptide gene expression in tiger shrimp, Penaeus monodon in response to gram-positive bacterial probionts and white spot virus challenge. Aquaculture 316, 6–12. https://doi.org/10.1016/j.aquaculture.2011.03.025 
  2. Antony, S.P., Bright Singh, I.S., Philip, R., 2011b. Molecular characterization and phylogenetic analysis of a penaeidin-like antimicrobial peptide, Fi-penaeidin from Fenneropenaeus indicus. Aquaculture 319, 298–301. https://doi.org/10.1016/j.aquaculture.2011.06.011 
  3. Antony, S.P., Bright Singh, I.S., Sudheer, N.S., Vrinda, S., Priyaja, P., Philip, R., 2011c. Molecular characterization of a crustin-like antimicrobial peptide in the giant tiger shrimp, Penaeus monodon, and its expression profile in response to various immunostimulants and challenge with WSSV. Immunobiology 216, 184–194. https://doi.org/10.1016/j.imbio.2010.05.030 
  4. Antony, S.P., Philip, R., Joseph, V., Bright Singh, I.S., 2011d. Anti-lipopolysaccharide factor and crustin-III, the anti-white spot virus peptides in Penaeus monodon: Control of viral infection by up-regulation. Aquaculture 319, 11–17. https://doi.org/10.1016/j.aquaculture.2011.06.022 
  5. Jose, S., Jayesh, P., Mohandas, A., Philip, R., Bright Singh, I.S., 2011. Application of primary haemocyte culture of Penaeus monodon in the assessment of cytotoxicity and genotoxicity of heavy metals and pesticides. Mar. Environ. Res. 71, 169–177. https://doi.org/10.1016/j.marenvres.2010.12.008 
  6. Kumar, V.J.R., Joseph, V., Vijai, R., Philip, R., Singh, I.S.B., 2011. Nitrification in a packed bed bioreactor integrated into a marine recirculating maturation system under different substrate concentrations and flow rates. J. Chem. Technol. Biotechnol. 86, 790–797. https://doi.org/10.1002/jctb.2588 
  7. Sreedharan, K., Rosamma, P., Bright Singh, I.S., 2011. Isolation and characterization of virulent Aeromonas veronii from ascitic fluid of oscar Astronotus ocellatus showing signs of infectious dropsy. Dis. Aquat. Organ. 94, 29–39. https://doi.org/10.3354/dao02304 
  8. Sudheer, N.S., Philip, R., Bright Singh, I.S., 2011. In vivo screening of mangrove plants for anti WSSV activity in Penaeus monodon, and evaluation of Ceriops tagal as a potential source of antiviral molecules. Aquaculture 311, 36–41. https://doi.org/10.1016/j.aquaculture.2010.11.016
  1. Antony, S.P., Bright Singh, I.S., Philip, R., 2010. Molecular characterization of a crustin-like, putative antimicrobial peptide, Fi-crustin, from the Indian white shrimp, Fenneropenaeus indicus. Fish Shellfish Immunol. 28, 216–220. https://doi.org/10.1016/j.fsi.2009.10.013 
  2. Jose, S., Mohandas, A., Philip, R., Bright Singh, I.S., 2010. Primary hemocyte culture of Penaeus monodon as an in vitro model for white spot syndrome virus titration, viral and immune related gene expression and cytotoxicity assays. J. Invertebr. Pathol. 105, 312–321. https://doi.org/10.1016/j.jip.2010.08.006 
  3. Pramod, Padinhare Kattil, Ramachandran, A., Sajeevan, T.P., Thampy, S., Pai, S.S., 2010. Comparative efficacy of MS-222 and benzocaine as anaesthetics under simulated transport conditions of a tropical ornamental fish Puntius filamentosus (Valenciennes). Aquac. Res. 41, 309–314. https://doi.org/10.1111/j.1365-2109.2009.02333.x 
  4. Pramod, P. K., Sajeevan, T.P., Ramachandran, A., Thampy, S., Pai, S.S., 2010. Effects of Two Anesthetics on Water Quality during Simulated Transport of a Tropical Ornamental Fish, the Indian tiger barb Puntius filamentosus. N. Am. J. Aquac. 72, 290–297. https://doi.org/10.1577/A09-063.1 
  5. Rejish Kumar, V.J., Joseph, V., Philip, R., Bright Singh, I.S., 2010. Nitrification in brackish water recirculating aquaculture system integrated with activated packed bed bioreactor. Water Sci. Technol. 61, 797. https://doi.org/10.2166/wst.2010.849 
  6. Sajeevan, T.P., Selven, S., Philip, R., 2010. β-Mercapto-ethanol-treated yeast showed better protection against white spot syndrome virus infection in Indian white shrimp Fenneropenaeus indicus. Aquac. Res. 41, e715–e718. https://doi.org/10.1111/j.1365-2109.2010.02547.x 
  7. Sukumaran, V., Lowman, D.W., Sajeevan, T.P., Philip, R., 2010. Marine yeast glucans confer better protection than that of baker’s yeast in Penaeus monodon against white spot syndrome virus infection. Aquac. Res. 41, 1799–1805. https://doi.org/10.1111/j.1365-2109.2010.02520.x 
  1. Anas, A., Lowman, D.W., Williams, D.L., Millen, S., Somnath Pai, S., Sajeevan, T.P., Philip, R., Bright Singh, I.S., 2009. Alkali insoluble glucan extracted from Acremonium diospyri is a more potent immunostimulant in the Indian White Shrimp, Fenneropenaeus indicus than alkali soluble glucan. Aquac. Res. 40, 1320–1327. https://doi.org/10.1111/j.1365-2109.2009.02231.x 
  2. Arnold, S.J., Coman, F.E., Jackson, C.J., Groves, S.A., 2009. High-intensity, zero water-exchange production of juvenile tiger shrimp, Penaeus monodon: An evaluation of artificial substrates and stocking density. Aquaculture 293, 42–48. https://doi.org/10.1016/j.aquaculture.2009.03.049 
  3. Manju, N.J., Deepesh, V., Achuthan, C., Philip, R., Bright Singh, I.S., 2009. Immobilization of nitrifying bacterial consortia on wood particles for bioaugmenting nitrification in shrimp culture systems. Aquaculture 294, 65–75. https://doi.org/10.1016/j.aquaculture.2009.05.008 
  4. Manjusha, M., Varghese, R., Philip, R., Mohandas, A., Bright Singh, I.S., 2009. Pathological changes in Fenneropenaeus indicus experimentally infected with white spot virus and virus morphogenesis. J. Invertebr. Pathol. 102, 225–232. https://doi.org/10.1016/j.jip.2009.08.011 
  5. Preetha, R., Jose, S., Prathapan, S., Vijayan, K.K., Jayaprakash, N.S., Philip, R., Bright Singh, I.S., 2009. An inhibitory compound produced by Pseudomonas with effectiveness on Vibrio harveyi. Aquac. Res. 41, 1452–1461. https://doi.org/10.1111/j.1365-2109.2009.02436.x
  6. Rejish Kumar, V.J., Achuthan, C., Manju, N.J., Philip, R., Bright Singh, I.S., 2009a. Mass production of nitrifying bacterial consortia for the rapid establishment of nitrification in saline recirculating aquaculture systems. World J. Microbiol. Biotechnol. 25, 407–414. https://doi.org/10.1007/s11274-008-9905-1 
  7. Rejish Kumar, V.J., Achuthan, C., Manju, N.J., Philip, R., Bright Singh, I.S., 2009b. Activated packed bed bioreactor for rapid nitrification in brackish water hatchery systems. J. Ind. Microbiol. Biotechnol. 36, 355–365. https://doi.org/10.1007/s10295-008-0504-9 
  8. Rejish Kumar, V.J., Achuthan, C., Manju, N.J., Philip, R., Bright Singh, I.S., 2009c. Stringed bed suspended bioreactors (SBSBR) for in situ nitrification in penaeid and non-penaeid hatchery systems. Aquac. Int. 17, 479–489. https://doi.org/10.1007/s10499-008-9218-2 
  9. Sajeevan, Thavarool Puthiyedathu, Lowman, D.W., Williams, D.L., Selven, S., Anas, A., Philip, R., Rosamma, P., 2009. Marine yeast diet confers better protection than its cell wall component (1-3)-β-d-glucan as an immunostimulant in Fenneropenaeus indicus. Aquac. Res. 40, 1723–1730. https://doi.org/10.1111/j.1365-2109.2009.02275.x
  10. Sajeevan, T.P., Philip, R., 2009. Effect of treatment with b-1,3 glucan from the yeast Candida sake S165 on protection to Fenneropenaeus indicus postlarvae against WSSV infection. J. Mar. Biol. Assoc. India 51, 227–230.
  11. Sajeevan, T. P., Philip, R., Bright Singh, I.S., 2009. Dose/frequency: A critical factor in the administration of glucan as immunostimulant to Indian white shrimp Fenneropenaeus indicus. Aquaculture 287, 248–252. https://doi.org/10.1016/j.aquaculture.2008.10.045 
  12. Somnath Pai, S., Anas, A., Jayaprakash, N.S., Priyaja, P., Sreelakshmi, B., Preetha, R., Philip, R., Mohandas, A., Bright Singh, I.S., 2009a. Penaeus monodon larvae can be protected from Vibrio harveyi infection by pre-emptive treatment of a rearing system with antagonistic or non-antagonistic bacterial probiotics. Aquac. Res. 41, 847–860. https://doi.org/10.1111/j.1365-2109.2009.02362.x 
  13. Somnath Pai, S., Manjusha, M., Anas, A., Ranjit, S., Sudheer, N.S., Philip, R., Bright Singh, I.S., 2009b. A survey of potential carriers and reservoir of White Spot Syndrome Virus in Penaeus monodon culture systems of Kerala. Indian J. Virol. 20.
  1. Anas, A., Philip, R., Bright Singh, I.S., 2008. Chitosan as a wall material for a microencapsulated delivery system for Macrobrachium rosenbergii (de Man) larvae. Aquac. Res. 39, 885–890. https://doi.org/10.1111/j.1365-2109.2008.01944.x 
  1. Preetha, R., Jayaprakash, N.S., Bright Singh, I.S., 2007a. Synechocystis MCCB 114 and 115 as putative probionts for Penaeus monodon post-larvae. Dis. Aquat. Organ. 74, 243–247. https://doi.org/10.3354/dao074243 
  2. Preetha, R., Jayaprakash, N.S., Philip, R., Bright Singh, I.S., 2007b. Optimization of carbon and nitrogen sources and growth factors for the production of an aquaculture probiotic (Pseudomonas MCCB 103) using response surface methodology. J. Appl. Microbiol. 102, 1043–1051. https://doi.org/10.1111/j.1365-2672.2006.03149.x 
  3. Preetha, R., Jayaprakash, N.S., Philip, R., Bright Singh, I.S., 2007c. Optimization of medium for the production of a novel aquaculture probiotic,Micrococcus MCCB 104 using central composite design. Biotechnol. Bioprocess Eng. 12, 548–555. https://doi.org/10.1007/BF02931353 
  1. Achuthan, C., Rejish Kumar, V.J., Manju, N.J., Philip, R., Bright Singh, I.S., 2006. Development of nitrifying bacterial consortia for immobilizing in nitrifying bioreactors designed for penaeid and non-penaeid larval rearing systems in the tropics. Indian J. Mar. Sci. 35, 240–248.
  2. Amar, B., Philip, R., Bright Singh, I.S., 2006. Efficacy of fermented prawn shell waste as a feed ingredient for Indian white prawn, Fenneropenaeus indicus. Aquac. Nutr. 12, 433–442. https://doi.org/10.1111/j.1365-2095.2006.00445.x
  3. Jayaprakash, N.S., Rejish Kumar, V.J., Philip, R., Bright Singh, I.S., 2006a. Vibrios associated with Macrobrachium rosenbergii (De Man, 1879) larvae from three hatcheries on the Indian southwest coast. Aquac. Res. 37, 351–358. https://doi.org/10.1111/j.1365-2109.2006.01432.x
  4. Jayaprakash, N.S., Somnath Pai, S., Philip, R., Bright Singh, I.S., 2006b. Isolation of a pathogenic strain of Vibrio alginolyticus from necrotic larvae of Macrobrachium rosenbergii (de Man). J. Fish Dis. 29, 187–191. https://doi.org/10.1111/j.1365-2761.2006.00690.x
  5. Mani, S.K., Kanjur, R., Bright Singh, I.S., Reed, R.H., 2006. Comparative effectiveness of solar disinfection using small-scale batch reactors with reflective, absorptive and transmissive rear surfaces. Water Res. 40, 721–727. https://doi.org/10.1016/j.watres.2005.11.039
  6. Sahul Hameed, A.S.S., Parameswaran, V., Shukla, R., Bright Singh, I.S., Thirunavukkarasu, A.R.R., Bhonde, R.R.R., 2006. Establishment and characterization of India’s first marine fish cell line (SISK) from the kidney of sea bass (Lates calcarifer). Aquaculture 257, 92–103. https://doi.org/10.1016/j.aquaculture.2006.01.011
  7. Sajeevan, T.P., Philip, R., Bright Singh, I.S., 2006. Immunostimulatory effect of a marine yeast Candida sake S165 in Fenneropenaeus indicus. Aquaculture 257, 150–155. https://doi.org/10.1016/j.aquaculture.2006.03.008
  8. Vijayan, K.K., Bright Singh, I.S., Jayaprakash, N.S., Alavandi, S. V., Somnath Pai, S., Preetha, R., Rajan, J.J.S., Santiago, T.C., 2006. A brackishwater isolate of Pseudomonas PS-102, a potential antagonistic bacterium against pathogenic vibrios in penaeid and non-penaeid rearing systems. Aquaculture 251, 192–200. https://doi.org/10.1016/j.aquaculture.2005.10.010
  1. Anas, A., Paul, S., Jayaprakash, N.S., Philip, R., Bright Singh, I.S., 2005. Antimicrobial activity of chitosan against vibrios from freshwater prawn Macrobrachium rosenbergii larval rearing systems. Dis. Aquat. Organ. 67, 177–179. https://doi.org/10.3354/dao067177
  2. Bright Singh, I.S., Manjusha, M., Somnath Pai, S., Philip, R., Bright Singh, I.S., 2005. Fenneropenaeus indicus is protected from white spot disease by oral administration of inactivated white spot syndrome virus. Dis. Aquat. Organ. 66, 265–270. https://doi.org/10.3354/dao066265
  3. Jayaprakash, N.S., Somnath Pai, S., Anas, A., Preetha, R., Philip, R., Bright Singh, I.S., 2005. A marine bacterium, Micrococcus MCCB 104, antagonistic to vibrios in prawn larval rearing systems. Dis. Aquat. Organ. 68, 39–45. https://doi.org/10.3354/dao068039
  1. Ajitha, S., Sridhar, M., Bright Singh, I.S., Varghese, V., 2004. Probiotic Effects of Lactic Acid Bacteria gainst Vibrio Alginolyticus in Penaeus (Fenneropenaeus) indicus (H. Milne Edwards). Asian Fish. Sci. 17, 71–80
  2. Ramachandran, K., Bright Singh, I.S., 2004. Optimum growth requirements of nitrifying consortia developed from treated sewage. Indian J. Exp. Biol. 42, 314–318.
  1. Anas, A., Bright Singh, I.S., 2003. Application of yeast glucan and bacterins for enhanced seed production of Macrobrachium rosenbergii (de Man). J. Aquac. Trop. 18.
  1. Nambudiri, D.D., George, S., Bright Singh, I.S., Sherief, P.M., 2001. Influence of storage conditions on the survival period of the clam Meretrix casta. J. Food Sci. Technol. 38, 379–380.
  2. Sunil Kumar, G., Bright Singh, I.S., Philip, R., 2001. Development of a cell culture system from the ovarian tissue of African catfish (Clarias gariepinus). Aquaculture 194, 51–62. https://doi.org/10.1016/S0044-8486(00)00509-3
  1. Philip, R., Sunil Kumar, G., Bright Singh, I.S., 2000. Development of cell culture system from liver, kidney, and spleen of African catfish, Clarias gariepinus. Vitr. – Cell. Dev. Biol. 36, 84.
  2. Vici, V., Bright Singh, I.S., Bhat, S.G., 2000. Application of bacterins and yeast Acremonium dyosporii to protect the larvae of Macrobrachium rosenbergii from vibriosis. Fish Shellfish Immunol. 10, 559–563. https://doi.org/10.1006/fsim.2000.0278 
  1. Bright Singh, I.S., Lakshmanaperumalsamy, P., Chandramohan, D., 1998. Bacterial flora of pond reared Penaeus indicus (Milne Edwards). J. Aquac. Trop. 13, 133–142.
  2. Bright Singh, I.S., Lakshmanaperumalsamy, P., Chandramohan, D., 1981. Finrot diseases in Etroplus suratensis. Bull. Dep. Mar. Sci. Univ. Cochin 12, 147–154.
  3. Bright Singh, I.S., Lakshmanaperumalsamy, P., Thomas, I., Chandrasekharan, M., Chandramohan, D., 1982. Bacteria associated with brown spot disease in Penaeus indicus H. Milne Edwards. Bull. Dep. Mar. Sci. Univ. Cochin.
  4. Bright Singh, I.S., Philip, R., 1995. A simple device for the separation of weak larvae of Macrobrachium rosenbergii (De Man). Aquac. Res. 26, 225–227. https://doi.org/10.1111/j.1365-2109.1995.tb00906.x
  5. Bright Singh, I.S., Philip, R., 1993. Comparative efficiency of three media in isolating heterotrophic bacteria from the larvae and larval rearing water of Macrobrachium rosenbergii. Indian J. Microbiol. 33, 67–69.
  6. Bright Singh, I.S., Philip, R., Maqbool, T.K., Ramesh, S., 1994. Bacteria associated with Epizootic Ulcerative Syndrome in fishes on inland waters of Kerala. Indian J. Microbiol. 34, 233. 
  7. Bright Singh, I.S., Philip, R., Raveendranath, M., Shanmugam, J., 1995. Development of primary cell cultures from kidney of freshwater fish Heteropneustes fossilis. Indian J. Exp. Biol. 33, 595–599.
  8. Lakshmanaperumalsamy, P., Chandrasekaran, P.M., Bright Singh, I.S., Chandramohan, D., 1981. Microbial indicators and pathogens near the mouth region of Vembanad Lake. Bull. Dep. Mar. Sci. Univ. Cochin 12, 103–109.
  9. Nambudiri, D.D., Bright Singh, I.S., George, S., Sherief, P.M., Maniappan, C., Ayoob, T.M., 1995. Design, fabrication and standardisation of a depuration system for bivalves. Fish. Technol. 32., 126-130.
  10. Sen, A., Bright Singh, I.S., Rengarajan, R., Philip, R., Kumar, G.S., Sen, A., 1999. Evidence of a Bacilliform Virus Causing Outbreaks of Whitespot Disease in Penaeus monodon H. Milne Edwards in India. Asian Fish. Sci. 12, 41–47.
  11. Sunil Kumar, G., Bright Singh, I.S., Philip, R., Raveendranath, M., Shanmugam, J., 1998. Efficacy of fish and prawn muscle extracts as supplements of medium in development of a primary cell culture system from larval tissue of aquarium fish Poecilia reticulata. Indian J. Exp. Biol. 36, 91–94.