Polyhydroxyalkanoates from Marine Microorganisms for Biomedical and Aquaculture Applications
Summary
Over 2500 bacterial isolates have been obtained from various marine samples and were screened for Polyhydroxyalkanoate (PHA) production. Isolates with >50% PHA production were further screened based on % PHA productivity. 27 isolates were identified by 16S rRNA gene sequencing and were subsequently deposited in GenBank. Four potent isolates Vibrio harveyi MCCB284, Bacillus cereus MCCB281, Labrenzia aggregata MCCB274, Vibrio alginolyticus MCCB290 from different locations were studied for PHA production, optimization and characterization studies. Polyhydroxybutyrate (PHB) homopolymer as well as polyhydroxybutyrate-co-hydroxyvalerate (PHBV) copolymer production was optimized and the polymers were purified and characterized. Optimization of copolymer production from Bacillus cereus was carried out using Response Surface Methodology with Design Expert software. Ability of 4 bacterial isolates Vibrio harveyi MCCB284, Bacillus cereus MCCB281 to produce PHA using glycerol as carbon source can be exploited for the sustainable commercial production of PHA. Nanoparticle synthesis using PHB and PHBV has been optimized and Nile Red was used as sample drug. PHBV film synthesis and characterization have been carried out for use in biomedical applications. Man Power- Two Ph.D. Scholars have been associated with the project.
Sampling at Goan Coast
TEM Micrograph showing PHA granules in Bacillus cereus MCCB281
SEM Micrograph showing PHA nanoparticles
Response surface 3D plots showing interactive effect of process variables for PHA yield
L929 fibroblast cells on the surface of (a) PHBV and (c) PHB films and after staining with Hoechst 33258 dye on (b) PHBV and (d) PHB films. DOI: 10.1016/j.ijbiomac.2021.02.006
Funding Agency
MoES-CMLRE
File No: MoES/CMLRE 10-MLR-TD/09/2012 dated 09/10/2012
Period: 2012-2017
Project Cost: 87 Lakhs
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